Analysis of cDNA and genomic clones coding for the pro alpha 1 chain of calf type II collagen.
Nucleic Acids Res
; 13(8): 2815-26, 1985 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-2582365
A bovine cDNA library constructed from fetal cartilage RNA was screened with a pro alpha 1(II) collagen specific chicken cDNA. A recombinant clone (Bc 7), with an insert of 1 kb, was identified and shown to contain sequences exhibiting 85% homology with the chicken pro alpha 1(II) collagen C-propeptide. Interspecies comparison strongly suggested that one potential glycosylation site present in the avian C-propeptide is not utilized, since this site is absent in the bovine chain. In addition, two overlapping genomic clones (Pal 3 and Pal 4) were isolated and partially characterized. These clones span 23 kb of DNA and contain approximately 17 kb of the pro alpha 1(II) calf gene. Sequencing of exon 1 has determined the length of the 3' untranslated region and the exact location of the polyadenylation attachment site.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Colágeno
Límite:
Animals
Idioma:
En
Revista:
Nucleic Acids Res
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Reino Unido