Assessment of affective and somatic signs of ethanol withdrawal in C57BL/6J mice using a short-term ethanol treatment.

Perez, E E; De Biasi, M

Perez, E E; De Biasi, M.

Afiliación

Perez EE; Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
De Biasi M; Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: marielde@mail.med.upenn.edu.

Alcohol ; 49(3): 237-43, 2015 May.

Article en En | MEDLINE | ID: mdl-25817777

RESUMEN

Alcohol is one of the most prevalent addictive substances in the world. Withdrawal symptoms result from abrupt cessation of alcohol consumption in habitual drinkers. The emergence of both affective and physical symptoms produces a state that promotes relapse. Mice provide a preclinical model that could be used to study alcohol dependence and withdrawal while controlling for both genetic and environmental variables. The use of a liquid ethanol diet offers a reliable method for the induction of alcohol dependence in mice, but this approach is impractical when conducting high-throughput pharmacological screens or when comparing multiple strains of genetically engineered mice. The goal of this study was to compare withdrawal-associated behaviors in mice chronically treated with a liquid ethanol diet vs. mice treated with a short-term ethanol treatment that consisted of daily ethanol injections containing the alcohol dehydrogenase inhibitor, 4-methylpyrazole. Twenty-four hours after ethanol treatment, mice were tested in the open field arena, the elevated plus maze, the marble burying test, or for changes in somatic signs during spontaneous ethanol withdrawal. Anxiety-like and compulsive-like behaviors, as well as physical signs, were all significantly elevated in mice undergoing withdrawal, regardless of the route of ethanol administration. Therefore, a short-term ethanol treatment can be utilized as a screening tool for testing genetic and pharmacological agents before investing in a more time-consuming ethanol treatment.

Asunto(s)

Afecto/efectos de los fármacos; Ansiedad/psicología; Conducta Animal/efectos de los fármacos; Depresores del Sistema Nervioso Central/farmacología; Conducta Compulsiva/psicología; Etanol/farmacología; Síndrome de Abstinencia a Sustancias/psicología; Animales; Ansiedad/inducido químicamente; Depresores del Sistema Nervioso Central/efectos adversos; Conducta Compulsiva/inducido químicamente; Etanol/efectos adversos; Femenino; Aseo Animal/efectos de los fármacos; Masculino; Masticación/efectos de los fármacos; Ratones; Ratones Endogámicos C57BL; Síndrome de Abstinencia a Sustancias/etiología; Síndrome de Abstinencia a Sustancias/fisiopatología; Vocalización Animal/efectos de los fármacos

Palabras clave

Alcohol; Anxiety-like behavior; Compulsive-like behavior; Mouse; Somatic signs; Withdrawal

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ansiedad / Síndrome de Abstinencia a Sustancias / Conducta Animal / Depresores del Sistema Nervioso Central / Conducta Compulsiva / Afecto / Etanol Tipo de estudio: Diagnostic_studies / Etiology_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Alcohol Asunto de la revista: TRANSTORNOS RELACIONADOS COM SUBSTANCIAS Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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