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A study of lymphoid organs and serum proinflammatory cytokines in pigs infected with African swine fever virus genotype II.
Zakaryan, Hovakim; Cholakyans, Victorya; Simonyan, Lusine; Misakyan, Alla; Karalova, Elena; Chavushyan, Andranik; Karalyan, Zaven.
Afiliación
  • Zakaryan H; Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, 0014, Yerevan, Armenia.
Arch Virol ; 160(6): 1407-14, 2015 Jun.
Article en En | MEDLINE | ID: mdl-25797197
African swine fever virus (ASFV), the causative agent of one of the most important viral diseases of domestic pigs for which no vaccine is available, causes immune system disorders in infected animals. In this study, the serum levels of proinflammatory cytokines, as well as the histological and cellular constitution of lymphoid organs of pigs infected with ASFV genotype II were investigated. The results showed a high degree of lymphocyte depletion in the lymphoid organs, particularly in the spleen and lymph nodes, where ASFV infection led to a twofold decrease in the number of lymphocytes on the final day of infection. Additionally, ASFV-infected pigs had atypical forms of lymphocytes found in all lymphoid organs. In contrast to lymphocytes, the number of immature immune cells, particularly myelocytes, increased dramatically and reached a maximum on day 7 postinfection. The serum levels of TNF-α, IL-1ß, IL-6, and IL-8 were evaluated. Proinflammatory cytokines showed increased levels after ASFV infection, with peak values at 7 days postinfection, and this highlights their role in the pathogenesis of ASFV. In conclusion, this study showed that ASFV genotype II, like other highly virulent strains, causes severe pathological changes in the immune system of pigs.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citocinas / Fiebre Porcina Africana / Virus de la Fiebre Porcina Africana / Tejido Linfoide Idioma: En Revista: Arch Virol Año: 2015 Tipo del documento: Article País de afiliación: Armenia Pais de publicación: Austria

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citocinas / Fiebre Porcina Africana / Virus de la Fiebre Porcina Africana / Tejido Linfoide Idioma: En Revista: Arch Virol Año: 2015 Tipo del documento: Article País de afiliación: Armenia Pais de publicación: Austria