Your browser doesn't support javascript.
loading
Profiling of small RNA cargo of extracellular vesicles shed by Trypanosoma cruzi reveals a specific extracellular signature.
Fernandez-Calero, Tamara; Garcia-Silva, Rosa; Pena, Alvaro; Robello, Carlos; Persson, Helena; Rovira, Carlos; Naya, Hugo; Cayota, Alfonso.
Afiliación
  • Fernandez-Calero T; Bioinformatics Unit, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo 11400, Uruguay.
  • Garcia-Silva R; Functional Genomics Unit, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo 11400, Uruguay.
  • Pena A; Bioinformatics Unit, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo 11400, Uruguay.
  • Robello C; Molecular Biology Unit, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo 11400, Uruguay.
  • Persson H; Division of Oncology, Department of Clinical Sciences, Lund University Cancer Center, Lund 223 81, Sweden.
  • Rovira C; Division of Oncology, Department of Clinical Sciences, Lund University Cancer Center, Lund 223 81, Sweden.
  • Naya H; Bioinformatics Unit, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo 11400, Uruguay.
  • Cayota A; Functional Genomics Unit, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo 11400, Uruguay; Department of Medicine, Faculty of Medicine, Universidad de la República, Av Gral Flores 2125, Montevideo 11800, Uruguay. Electronic address: cayota@pasteur.edu.uy.
Mol Biochem Parasitol ; 199(1-2): 19-28, 2015.
Article en En | MEDLINE | ID: mdl-25795082
Over the last years, an expanding family of small regulatory RNAs (e.g. microRNAs, siRNAs and piRNAs) was recognized as key players in novel forms of post-transcriptional gene regulation in most eukaryotes. However, the machinery associated with Ago/Dicer-dependent small RNA biogenesis was thought to be either entirely lost or extensively simplified in some unicellular organisms including Trypanosoma cruzi, Saccharomyces cerevisiae, Leishmania major and Plasmodium falciparum. Although the biogenesis of small RNAs from non-coding RNAs represent a minor fraction of the normal small RNA transcriptome in eukaryotic cells, they represent the unique small RNA pathways in Trypanosoma cruzi which produce different populations of small RNAs derived from tRNAs, rRNAs, sn/snoRNAs and mRNAs. These small RNAs are secreted included in extracellular vesicles and transferred to other parasites and susceptible mammalian cells. This process represents a novel form of cross-kingdom transfer of genetic material suggesting that secreted vesicles could represent new relevant pieces in life cycle transitions, infectivity and cell-to-cell communication. Here, we provide for the first time a detailed analysis of the small RNA cargo of extracellular vesicles from T. cruzi epimastigotes under nutritional stress conditions compared to the respective intracellular compartment using deep sequencing. Compared with the intracellular compartment, shed extracellular vesicles showed a specific extracellular signature conformed by distinctive patterns of small RNAs derived from rRNA, tRNA, sno/snRNAs and protein coding sequences which evidenced specific secretory small RNA processing pathways.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma cruzi / ARN Protozoario / Vesículas Extracelulares Idioma: En Revista: Mol Biochem Parasitol Año: 2015 Tipo del documento: Article País de afiliación: Uruguay Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma cruzi / ARN Protozoario / Vesículas Extracelulares Idioma: En Revista: Mol Biochem Parasitol Año: 2015 Tipo del documento: Article País de afiliación: Uruguay Pais de publicación: Países Bajos