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Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes.
German, Sergio D; Lee, Joon-Hee; Campbell, Keith H; Sweetman, Dylan; Alberio, Ramiro.
Afiliación
  • German SD; Division of Animal Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom.
  • Lee JH; Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Republic of Korea Institute of Agriculture & Life Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
  • Campbell KH; Division of Animal Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom.
  • Sweetman D; Division of Animal Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom dylan.sweetman@nottingham.ac.uk.
  • Alberio R; Division of Animal Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom ramiro.alberio@nottingham.ac.uk.
Biol Reprod ; 92(4): 103, 2015 Apr.
Article en En | MEDLINE | ID: mdl-25788662
Oocytes treated with the protein synthesis inhibitor cycloheximide (CHX) arrest at the germinal vesicle (GV) stage and undergo accelerated GV breakdown (GVBD) after CHX is removed. However, little is known about the underlying mechanism of accelerated meiotic maturation. Here, we investigated this mechanism and found that oocytes released from CHX arrest have higher amounts of cyclin B1 (CCNB1) and phosphorylated mitogen-activated protein kinase (pMAPK) proteins. Increased levels of these factors were not associated with mRNA polyadenylation or increased transcription rates of CCNB1 and MOS (Moloney murine sarcoma viral oncogene homolog) during CHX arrest. We found that treatment of CHX-arrested oocytes with the actin filament-stabilizing agent Jasplakinolide (Jasp) delayed GVBD following release from CHX arrest and that this was correlated with reduced maturation-promoting factor (MPF) activity. These results suggest that CCNB1 mRNAs released from actin filaments during CHX arrest increase CCNB1 transcripts available for translation after release from CHX arrest, leading to the precocious activation of MPF and accelerated meiotic progression.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Inhibidores de la Síntesis de la Proteína / Actinas / Cicloheximida / Meiosis Tipo de estudio: Risk_factors_studies Límite: Animals / Pregnancy Idioma: En Revista: Biol Reprod Año: 2015 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Inhibidores de la Síntesis de la Proteína / Actinas / Cicloheximida / Meiosis Tipo de estudio: Risk_factors_studies Límite: Animals / Pregnancy Idioma: En Revista: Biol Reprod Año: 2015 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos