[Detection of Plasmodium falciparum by using magnetic nanoparticles separation-based quantitative real-time PCR assay].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
; 26(5): 522-5, 530, 2014 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-25782249
OBJECTIVE: To establish a magnetic nanoparticles separation-based quantitative real-time PCR (RT-PCR) assay for fast and accurate detection of Plasmodium falciparum and providing a technical support for improving the control and prevention of imported malaria. METHODS: According to the conserved sequences of the P. falciparum genome 18SrRNA, the species-specific primers and probe were designed and synthetized. The RT-PCR was established by constructing the plasmid standard, fitting the standard curve and using magnetic nanoparticles separation. The sensitivity and specificity of the assay were evaluated. RESULTS: The relationship between the threshold cycle (Ct) and logarithm of initial templates copies was linear over a range of 2.5 x 10(1) to 2.5 x 10(8) copies/µl (R2 = 0.999). Among 13 subjects of entry frontier, a P. falciparum carrier with low load was detected by using the assay and none was detected with the conventional examinations (microscopic examinations and rapid tests). CONCLUSION: This assay shows a high sensitivity in detection of P. falciparum, with rapid and accurate characteristics, and is especially useful in diagnosis of P. falciparum infectors with low parasitaemia at entry-exit frontier ports.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plasmodium falciparum
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Malaria
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
Año:
2014
Tipo del documento:
Article
Pais de publicación:
China