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The Effect of IL-22 and IL-28 in Induction of Type 1 Regulatory T (Tr1) Cells.
Arasteh, Javad; Ebtekar, Massoumeh; Pourpak, Zahra; Pourfatollah, Ali Akbar; Hassan, Zuhair Mohammad; Kardar, Gholam Ali; Zare, Ahad; Saghafi, Shiva; Tabar Molla Hassan, Agheel.
Afiliación
  • Arasteh J; Department of Biology, Faculty of Basic Sciences, Islamic Azad University Central Tehran Branch, Tehran, Iran araste_j@yahoo.com.
  • Ebtekar M; Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran araste_j@yahoo.com.
  • Pourpak Z; Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran zpourpak@yahoo.com.
  • Pourfatollah AA; Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran pourfa@yahoo.com.
  • Hassan ZM; Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran araste_j@yahoo.com.
  • Kardar GA; Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran araste_j@yahoo.com.
  • Zare A; Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran araste_j@yahoo.com.
  • Saghafi S; Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran araste_j@yahoo.com.
  • Tabar Molla Hassan A; Department of Immunology, Faculty of Medical Sciences,, Islamic Azad University Babol Branch, Babol, Iran araste_j@yahoo.com.
Iran J Allergy Asthma Immunol ; 14(2): 158-67, 2015 Apr.
Article en En | MEDLINE | ID: mdl-25780882
Cytokines have been introduced as critical inducers in the development of Th subpopulations.Cytokines like IL-10 are involved in inducing regulatory T cells such as Type 1 regulatory T (Tr1) cells cells. IL-22 is a member of IL-10 family of cytokines, and IL-28A is a member of IFN-γ family. In this study, cord blood mononuclear cells (CBMC) from normal healthy individuals were isolated by Ficoll and then naïve T cells were purified by CD4+CD25+ Regulatory T cell Isolation kit. The effect of these two cytokines on production of IL-5, TGF-ß, IL-10, IL-4 and IFN-γ cytokines from cord blood T cells was investigated to identify Tr1 cells as well as Th1 and Th2 polarization. Flow cytometric analysis showed that IL-28A and IL-22 were not effective in expression of IL-5 and TGF-ß either alone or in synergy, but in view of IL-10, IL-4 and IFN-γ, the results showed that IL-22 increased IL-10 and IL-4 but had a decreasing effect on IFN-γ. The results showed that IL-28A was not effective in increasing or decreasing the level of IL-10, IL-4 and IFN-γ. Therefore, according to these results, IL-22 and IL-28A were not effective in inducing Tr1 cells.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Interleucinas / Linfocitos T Reguladores Límite: Humans Idioma: En Revista: Iran J Allergy Asthma Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Irán
Buscar en Google
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Interleucinas / Linfocitos T Reguladores Límite: Humans Idioma: En Revista: Iran J Allergy Asthma Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Irán