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Biomimetic collagen I and IV double layer Langmuir-Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells.
Sorkio, Anni E; Vuorimaa-Laukkanen, Elina P; Hakola, Hanna M; Liang, Huamin; Ujula, Tiina A; Valle-Delgado, Juan José; Österberg, Monika; Yliperttula, Marjo L; Skottman, Heli.
Afiliación
  • Sorkio AE; BioMediTech, University of Tampere FM5/BMT, 33014 University of Tampere, Finland. Electronic address: anni.sorkio@uta.fi.
  • Vuorimaa-Laukkanen EP; Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, 33720 Tampere, Finland.
  • Hakola HM; Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, 33720 Tampere, Finland.
  • Liang H; Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, 33720 Tampere, Finland.
  • Ujula TA; Department of Forest Products Technology, School of Chemical Technology, Aalto University, P.O. Box 16300, 00076 Aalto, Finland.
  • Valle-Delgado JJ; Department of Forest Products Technology, School of Chemical Technology, Aalto University, P.O. Box 16300, 00076 Aalto, Finland.
  • Österberg M; Department of Forest Products Technology, School of Chemical Technology, Aalto University, P.O. Box 16300, 00076 Aalto, Finland.
  • Yliperttula ML; Division of Biopharmaceutical Sciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5, 00790 Helsinki, Finland.
  • Skottman H; BioMediTech, University of Tampere FM5/BMT, 33014 University of Tampere, Finland.
Biomaterials ; 51: 257-269, 2015 May.
Article en En | MEDLINE | ID: mdl-25771016
The environmental cues received by the cells from synthetic substrates in vitro are very different from those they receive in vivo. In this study, we applied the Langmuir-Schaefer (LS) deposition, a variant of Langmuir-Blodgett technique, to fabricate a biomimetic microenvironment mimicking the structure and organization of native Bruch's membrane for the production of the functional human embryonic stem cell derived retinal pigment epithelial (hESC-RPE) cells. Surface pressure-area isotherms were measured simultaneously with Brewster angle microscopy to investigate the self-assembly of human collagens type I and IV on air-subphase interface. Furthermore, the structure of the prepared collagen LS films was characterized with scanning electron microscopy, atomic force microscopy, surface plasmon resonance measurements and immunofluorescent staining. The integrity of hESC-RPE on double layer LS films was investigated by measuring transepithelial resistance and permeability of small molecular weight substance. Maturation and functionality of hESC-RPE cells on double layer collagen LS films was further assessed by RPE-specific gene and protein expression, growth factor secretion, and phagocytic activity. Here, we demonstrated that the prepared collagen LS films have layered structure with oriented fibers corresponding to architecture of the uppermost layers of Bruch's membrane and result in increased barrier properties and functionality of hESC-RPE cells as compared to the commonly used dip-coated controls.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colágeno Tipo I / Colágeno Tipo IV / Células Madre Pluripotentes / Materiales Biomiméticos / Epitelio Pigmentado de la Retina / Microambiente Celular Límite: Humans Idioma: En Revista: Biomaterials Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colágeno Tipo I / Colágeno Tipo IV / Células Madre Pluripotentes / Materiales Biomiméticos / Epitelio Pigmentado de la Retina / Microambiente Celular Límite: Humans Idioma: En Revista: Biomaterials Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos