Development of a time-resolved fluorescence probe for evaluation of competitive binding to the cholecystokinin 2 receptor.

Elshan, N G R Dayan; Jayasundera, Thanuja; Weber, Craig S; Lynch, Ronald M; Mash, Eugene A

Elshan, N G R Dayan; Jayasundera, Thanuja; Weber, Craig S; Lynch, Ronald M; Mash, Eugene A.

Afiliación

Elshan NG; Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721-0041, USA.
Jayasundera T; Department of Physiology, University of Arizona, Tucson, AZ 85724-5051, USA.
Weber CS; Department of Physiology, University of Arizona, Tucson, AZ 85724-5051, USA.
Lynch RM; Department of Physiology, University of Arizona, Tucson, AZ 85724-5051, USA; The Bio5 Institute, University of Arizona, Tucson, AZ 85721-0240, USA.
Mash EA; Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721-0041, USA. Electronic address: emash@email.arizona.edu.

Bioorg Med Chem ; 23(8): 1841-8, 2015 Apr 15.

Article en En | MEDLINE | ID: mdl-25769518

Asunto(s)

Colorantes Fluorescentes/química; Colorantes Fluorescentes/metabolismo; Receptor de Colecistoquinina B/metabolismo; Unión Competitiva; Colorantes Fluorescentes/síntesis química; Células HEK293; Humanos; Espectrometría de Fluorescencia

Palabras clave

Cholecystokinin 2 receptor; Dissociation-enhanced lanthanide fluoroimmunoassay; High-throughput screening; Multivalent binding; Time-resolved fluorescence

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptor de Colecistoquinina B / Colorantes Fluorescentes Límite: Humans Idioma: En Revista: Bioorg Med Chem Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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