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Two-photon imaging of cellular dynamics in the mouse spinal cord.
Weinger, Jason G; Greenberg, Milton L; Matheu, Melanie P; Parker, Ian; Walsh, Craig M; Lane, Thomas E; Cahalan, Michael D.
Afiliación
  • Weinger JG; Molecular Biology and Biochemistry, University of California, Irvine.
  • Greenberg ML; Physiology and Biophysics, University of California, Irvine.
  • Matheu MP; University of California San Francisco Diabetes Center, University of California, San Francisco.
  • Parker I; Neurobiology and Behavior, University of California, Irvine.
  • Walsh CM; Molecular Biology and Biochemistry, University of California, Irvine.
  • Lane TE; Pathology, University of Utah.
  • Cahalan MD; Physiology and Biophysics, University of California, Irvine; mcahalan@uci.edu.
J Vis Exp ; (96)2015 Feb 22.
Article en En | MEDLINE | ID: mdl-25742043
Two-photon (2P) microscopy is utilized to reveal cellular dynamics and interactions deep within living, intact tissues. Here, we present a method for live-cell imaging in the murine spinal cord. This technique is uniquely suited to analyze neural precursor cell (NPC) dynamics following transplantation into spinal cords undergoing neuroinflammatory demyelinating disorders. NPCs migrate to sites of axonal damage, proliferate, differentiate into oligodendrocytes, and participate in direct remyelination. NPCs are thereby a promising therapeutic treatment to ameliorate chronic demyelinating diseases. Because transplanted NPCs migrate to the damaged areas on the ventral side of the spinal cord, traditional intravital 2P imaging is impossible, and only information on static interactions was previously available using histochemical staining approaches. Although this method was generated to image transplanted NPCs in the ventral spinal cord, it can be applied to numerous studies of transplanted and endogenous cells throughout the entire spinal cord. In this article, we demonstrate the preparation and imaging of a spinal cord with enhanced yellow fluorescent protein-expressing axons and enhanced green fluorescent protein-expressing transplanted NPCs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Médula Espinal / Microscopía de Fluorescencia por Excitación Multifotónica / Células-Madre Neurales Límite: Animals Idioma: En Revista: J Vis Exp Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Médula Espinal / Microscopía de Fluorescencia por Excitación Multifotónica / Células-Madre Neurales Límite: Animals Idioma: En Revista: J Vis Exp Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos