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Nerve demyelination increases metabotropic glutamate receptor subtype 5 expression in peripheral painful mononeuropathy.
Ko, Miau-Hwa; Hsieh, Yu-Lin; Hsieh, Sung-Tsang; Tseng, To-Jung.
Afiliación
  • Ko MH; Department of Anatomy, College of Medicine, China Medical University, Taichung 40402, Taiwan. mhko@mail.cmu.edu.tw.
  • Hsieh YL; Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. ylhsieh@kmu.edu.tw.
  • Hsieh ST; Department of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei 10051, Taiwan. shsieh@ntu.edu.tw.
  • Tseng TJ; Department of Neurology, National Taiwan University Hospital, Taipei 10002, Taiwan. shsieh@ntu.edu.tw.
Int J Mol Sci ; 16(3): 4642-65, 2015 Mar 02.
Article en En | MEDLINE | ID: mdl-25739080
Wallerian degeneration or nerve demyelination, arising from spinal nerve compression, is thought to bring on chronic neuropathic pain. The widely distributed metabotropic glutamate receptor subtype 5 (mGluR5) is involved in modulating nociceptive transmission. The purpose of this study was to investigate the potential effects of mGluR5 on peripheral hypersensitivities after chronic constriction injury (CCI). Sprague-Dawley rats were operated on with four loose ligatures around the sciatic nerve to induce thermal hyperalgesia and mechanical allodynia. Primary afferents in dermis after CCI exhibited progressive decreases, defined as partial cutaneous denervation; importantly, mGluR5 expressions in primary afferents were statistically increased. CCI-induced neuropathic pain behaviors through the intraplantar injections of 2-methyl-6-(phenylethynyl)-pyridine (MPEP), a selective mGluR5 antagonist, were dose-dependently attenuated. Furthermore, the most increased mGluR5 expressions in primary afferents surrounded by reactive Schwann cells were observed at the distal CCI stumps of sciatic nerves. In conclusion, these results suggest that nerve demyelination results in the increases of mGluR5 expression in injured primary afferents after CCI; and further suggest that mGluR5 represents a main therapeutic target in developing pharmacological strategies to prevent peripheral hypersensitivities.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Desmielinizantes / Mononeuropatías / Receptor del Glutamato Metabotropico 5 / Neuralgia Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2015 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Desmielinizantes / Mononeuropatías / Receptor del Glutamato Metabotropico 5 / Neuralgia Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2015 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza