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c-erbB-2 amplification in node-negative human breast cancer.
Ro, J S; el-Naggar, A; Ro, J Y; Blick, M; Frye, D; Fraschini, G; Fritsche, H; Hortobagyi, G.
Afiliación
  • Ro JS; Department of Medical Oncology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Cancer Res ; 49(24 Pt 1): 6941-4, 1989 Dec 15.
Article en En | MEDLINE | ID: mdl-2573424
c-erbB-2 gene analysis by Southern and DNA dot blot methods was done in 66 tumor samples from patients with histologically node-negative breast cancer. The c-erbB-2 gene was amplified 2- to greater than 8-fold in 13 tumors (20%). None of 59 tumors that were examined by the Southern method showed c-erbB-2 gene rearrangement. c-erbB-2 amplification was analyzed in relation to other prognostic factors. The c-erbB-2 gene was amplified in five of 36 (14%) diploid and eight of 30 (27%) aneuploid tumors. Thirteen of 54 (24%) tumors with nuclear Grade 1 or 2 displayed c-erbB-2 amplification, whereas none of 12 tumors with nuclear Grade 3 did. No correlation was observed with estrogen receptor content, tumor size, histological type, or age of patients. The median follow-up date for these patients was 85+ mo. Of 13 patients whose tumors showed c-erbB-2 amplification, six patients (46%) developed recurrence, and five patients (38%) died of metastatic disease. In contrast, of 53 patients whose tumors did not show c-erbB-2 amplification, 15 patients (28%) developed recurrence, and seven patients (13%) died of disease. In conclusion, our results show that c-erbB-2 gene amplification was more frequent in aneuploid tumors and tumors with poor nuclear grade. c-erbB-2 amplification may be considered a possible prognostic factor in node-negative breast cancer.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proto-Oncogenes / Neoplasias de la Mama / Amplificación de Genes / Proteínas Proto-Oncogénicas Tipo de estudio: Prognostic_studies Límite: Adult / Aged / Aged80 / Female / Humans / Middle aged Idioma: En Revista: Cancer Res Año: 1989 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proto-Oncogenes / Neoplasias de la Mama / Amplificación de Genes / Proteínas Proto-Oncogénicas Tipo de estudio: Prognostic_studies Límite: Adult / Aged / Aged80 / Female / Humans / Middle aged Idioma: En Revista: Cancer Res Año: 1989 Tipo del documento: Article Pais de publicación: Estados Unidos