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A role for matrix stiffness in the regulation of cardiac side population cell function.
Qiu, Yiling; Bayomy, Ahmad F; Gomez, Marcus V; Bauer, Michael; Du, Ping; Yang, Yanfei; Zhang, Xin; Liao, Ronglih.
Afiliación
  • Qiu Y; Divisions of Genetics and Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts;
  • Bayomy AF; Divisions of Genetics and Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts; Department of Orthopedics and Sports Medicine, School of Medicine, University of Washington, Seattle, Washington; and.
  • Gomez MV; Divisions of Genetics and Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts;
  • Bauer M; Divisions of Genetics and Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts;
  • Du P; Department of Mechanical Engineering, Boston University, Boston, Massachusetts.
  • Yang Y; Divisions of Genetics and Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts;
  • Zhang X; Department of Mechanical Engineering, Boston University, Boston, Massachusetts.
  • Liao R; Divisions of Genetics and Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts; rliao@rics.bwh.harvard.edu.
Am J Physiol Heart Circ Physiol ; 308(9): H990-7, 2015 May 01.
Article en En | MEDLINE | ID: mdl-25724498
The mechanical properties of the local microenvironment may have important influence on the fate and function of adult tissue progenitor cells, altering the regenerative process. This is particularly critical following a myocardial infarction, in which the normal, compliant myocardial tissue is replaced with fibrotic, stiff scar tissue. In this study, we examined the effects of matrix stiffness on adult cardiac side population (CSP) progenitor cell behavior. Ovine and murine CSP cells were isolated and cultured on polydimethylsiloxane substrates, replicating the elastic moduli of normal and fibrotic myocardium. Proliferation capacity and cell cycling were increased in CSP cells cultured on the stiff substrate with an associated reduction in cardiomyogeneic differentiation and accelerated cell ageing. In addition, culture on stiff substrate stimulated upregulation of extracellular matrix and adhesion proteins gene expression in CSP cells. Collectively, we demonstrate that microenvironment properties, including matrix stiffness, play a critical role in regulating progenitor cell functions of endogenous resident CSP cells. Understanding the effects of the tissue microenvironment on resident cardiac progenitor cells is a critical step toward achieving functional cardiac regeneration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Miocitos Cardíacos / Mecanotransducción Celular / Dimetilpolisiloxanos / Células Madre Adultas / Nicho de Células Madre / Células de Población Lateral Límite: Animals Idioma: En Revista: Am J Physiol Heart Circ Physiol Asunto de la revista: CARDIOLOGIA / FISIOLOGIA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Miocitos Cardíacos / Mecanotransducción Celular / Dimetilpolisiloxanos / Células Madre Adultas / Nicho de Células Madre / Células de Población Lateral Límite: Animals Idioma: En Revista: Am J Physiol Heart Circ Physiol Asunto de la revista: CARDIOLOGIA / FISIOLOGIA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos