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MicroRNA profiling of the intestine during hypothermic circulatory arrest in swine.
Lin, Wei-Bin; Liang, Meng-Ya; Chen, Guang-Xian; Yang, Xiao; Qin, Han; Yao, Jian-Ping; Feng, Kang-Ni; Wu, Zhong-Kai.
Afiliación
  • Lin WB; Wei-Bin Lin, Meng-Ya Liang, Guang-Xian Chen, Xiao Yang, Han Qin, Jian-Ping Yao, Kang-Ni Feng, Zhong-Kai Wu, 2 Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
  • Liang MY; Wei-Bin Lin, Meng-Ya Liang, Guang-Xian Chen, Xiao Yang, Han Qin, Jian-Ping Yao, Kang-Ni Feng, Zhong-Kai Wu, 2 Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
  • Chen GX; Wei-Bin Lin, Meng-Ya Liang, Guang-Xian Chen, Xiao Yang, Han Qin, Jian-Ping Yao, Kang-Ni Feng, Zhong-Kai Wu, 2 Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
  • Yang X; Wei-Bin Lin, Meng-Ya Liang, Guang-Xian Chen, Xiao Yang, Han Qin, Jian-Ping Yao, Kang-Ni Feng, Zhong-Kai Wu, 2 Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
  • Qin H; Wei-Bin Lin, Meng-Ya Liang, Guang-Xian Chen, Xiao Yang, Han Qin, Jian-Ping Yao, Kang-Ni Feng, Zhong-Kai Wu, 2 Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
  • Yao JP; Wei-Bin Lin, Meng-Ya Liang, Guang-Xian Chen, Xiao Yang, Han Qin, Jian-Ping Yao, Kang-Ni Feng, Zhong-Kai Wu, 2 Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
  • Feng KN; Wei-Bin Lin, Meng-Ya Liang, Guang-Xian Chen, Xiao Yang, Han Qin, Jian-Ping Yao, Kang-Ni Feng, Zhong-Kai Wu, 2 Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
  • Wu ZK; Wei-Bin Lin, Meng-Ya Liang, Guang-Xian Chen, Xiao Yang, Han Qin, Jian-Ping Yao, Kang-Ni Feng, Zhong-Kai Wu, 2 Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
World J Gastroenterol ; 21(7): 2183-90, 2015 Feb 21.
Article en En | MEDLINE | ID: mdl-25717255
AIM: To perform a profiling analysis of changes in intestinal microRNA (miRNA) expression during hypothermic circulatory arrest (HCA). METHODS: A total of eight piglets were randomly divided into HCA and sham operation (SO) groups. Under general anesthesia, swine in the HCA group were subjected to hypothermic cardiopulmonary bypass at 24 °C followed by 80 min of circulatory arrest, and the reperfusion lasted for 180 min after cross-clamp removal. The counterparts in the SO group were only subjected to median sternotomy. Histopathological analysis was used to detect mucosal injury, and Pick-and-Mix custom miRNA real-time polymerase chain reaction (PCR) panels containing 306 unique primer sets were utilized to assay unpooled intestinal samples harvested from the two groups. RESULTS: The intestinal mucosa of the animals that were subjected to 24 °C HCA exhibited representative ischemic reperfusion injury of grade 2 or 3 according to the Chiu score. Such intestinal mucosal injuries, with the subepithelial space and epithelial layer lifting away from the lamina propria, were accompanied by shortened and irregular villi. On the contrary, the intestinal mucosa remained normal in the sham-operated animals. In total, twenty-five miRNAs were differentially expressed between the two groups (15 upregulated and 10 downregulated in the HCA group). Among these, eight miRNAs (miR-122, miR-221-5p, miR-31, miR-421-5p, miR-4333, miR-499-3p, miR-542 and let-7d-3p) were significantly dysregulated (four higher and four lower). The expression of miR-122 was significantly (5.37-fold) increased in the HCA group vs the SO group, indicating that it may play a key role in HCA-induced mucosal injury. CONCLUSION: Exposure to HCA caused intestinal miRNA dysregulation and barrier dysfunction in swine. These altered miRNAs might be related to the protection or destruction of the intestinal barrier.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión / Perfilación de la Expresión Génica / MicroARNs / Isquemia Mesentérica / Paro Cardíaco Inducido / Hipotermia Inducida / Mucosa Intestinal Tipo de estudio: Etiology_studies / Prognostic_studies Límite: Animals Idioma: En Revista: World J Gastroenterol Asunto de la revista: GASTROENTEROLOGIA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión / Perfilación de la Expresión Génica / MicroARNs / Isquemia Mesentérica / Paro Cardíaco Inducido / Hipotermia Inducida / Mucosa Intestinal Tipo de estudio: Etiology_studies / Prognostic_studies Límite: Animals Idioma: En Revista: World J Gastroenterol Asunto de la revista: GASTROENTEROLOGIA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos