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Expression and distribution of phosphodiesterase isoenzymes in the human male urethra.
Kedia, George T; Ückert, Stefan; Oelke, Matthias; Sonnenberg, Joachim E; Sohn, Michael; Kuczyk, Markus A; Hedlund, Petter.
Afiliación
  • Kedia GT; Department of Urology and Urological Oncology, Division of Surgery, Hannover Medical School, Hannover, Germany. Electronic address: kedia.george@mh-hannover.de.
  • Ückert S; Department of Urology and Urological Oncology, Division of Surgery, Hannover Medical School, Hannover, Germany; Urological Research Unit, Institute for Biochemical Research and Analysis, Barsinghausen am Deister, Germany.
  • Oelke M; Department of Urology and Urological Oncology, Division of Surgery, Hannover Medical School, Hannover, Germany.
  • Sonnenberg JE; Urological Research Unit, Institute for Biochemical Research and Analysis, Barsinghausen am Deister, Germany.
  • Sohn M; Department of Urology, AGAPLESION Markus Hospital, Frankfurt am Main, Germany.
  • Kuczyk MA; Department of Urology and Urological Oncology, Division of Surgery, Hannover Medical School, Hannover, Germany.
  • Hedlund P; Urological Research Institute, University Vita Salute San Raffaele, Milano, Italy; Department of Clinical Pharmacology, Faculty of Medicine, Linköping University, Linköping, Sweden.
Urology ; 85(4): 964.e1-6, 2015 Apr.
Article en En | MEDLINE | ID: mdl-25704994
OBJECTIVE: To investigate the expression and distribution of phosphodiesterase (PDE) isoenzymes PDE1A, PDE2A, PDE4A, PDE4B, and PDE5A in human urethral tissue. METHODS: Specimens of penile urethra were obtained from male subjects who had undergone male-to-female sex reassignment surgery. Using immunohistochemistry (immunofluorescence), the occurrence of PDE1A, PDE2A, PDE4A, PDE4B, and PDE5A, the neuronal nitric oxide synthase, calcitonin gene-related peptide, and vasoactive intestinal polypeptide was examined in urethral sections. Cytosolic supernatants prepared from isolated human urethral tissue were subjected to Western blot analysis using specific anti-PDE antibodies. RESULTS: Immunosignals specific for PDE1A, 4A, 4B, and 5A were observed in the urethral smooth musculature. The smooth muscle bundles were seen innervated by slender nerve fibers, characterized by the expression of the neuronal nitric oxide synthase, calcitonin gene-related peptide, and vasoactive intestinal polypeptide. The expression of the PDE isoenzymes mentioned was confirmed by Western blotting. CONCLUSION: The results provide evidence for a significance of both the cyclic adenosine monophosphate and cyclic guanosine monophosphate signaling in the control of human urethral smooth muscle. The selective inhibition of PDE isoenzymes might represent a pharmacologic option to influence the function of smooth musculature in the human outflow region.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Uretra / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 1 / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 2 / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 4 / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 5 / Músculo Liso Límite: Humans / Male / Middle aged Idioma: En Revista: Urology Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Uretra / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 1 / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 2 / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 4 / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 5 / Músculo Liso Límite: Humans / Male / Middle aged Idioma: En Revista: Urology Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos