High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column.
Food Chem
; 133(2): 489-96, 2012 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-25683424
A simple and rapid high performance liquid chromatographic with fluorescence detection method for the determination of the aflatoxin B1, B2, G1 and G2 in peanuts, rice and chilli was developed. The sample was extracted using acetonitrile:water (90:10, v/v%) and then purified by using ISOLUTE® multimode solid phase extraction. After the pre-column derivatisation, the analytes were separated within 3.7 min using Chromolith® performance RP-18e (100-4.6mm) monolithic column. To assess the possible effects of endogenous components in the food items, matrix-matched calibration was used for the quantification and validation. The recoveries of aflatoxins that were spiked into food samples were 86.38-104.5% and RSDs were <4.4%. The method was applied to the determination of aflatoxins in peanut (9), rice (5) and chilli (10) samples. Liquid chromatography-tandem mass spectrometry analysis using triple quadruple analyser and operated in the multiple reaction monitoring modes on the contaminated samples was performed for confirmation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arachis
/
Oryza
/
Capsicum
/
Cromatografía Líquida de Alta Presión
/
Aflatoxina B1
/
Aflatoxinas
Idioma:
En
Revista:
Food Chem
Año:
2012
Tipo del documento:
Article
País de afiliación:
Malasia
Pais de publicación:
Reino Unido