BMP signaling and microtubule organization regulate synaptic strength.

Ball, R W; Peled, E S; Guerrero, G; Isacoff, E Y

Ball, R W; Peled, E S; Guerrero, G; Isacoff, E Y.

Afiliación

Ball RW; Department of Molecular and Cell Biology and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, United States.
Peled ES; Department of Molecular and Cell Biology and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, United States.
Guerrero G; Department of Molecular and Cell Biology and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, United States.
Isacoff EY; Department of Molecular and Cell Biology and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, United States; Physical Bioscience Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States. Electronic address: ehud@berkeley.edu.

Neuroscience ; 291: 155-66, 2015 Apr 16.

Article en En | MEDLINE | ID: mdl-25681521

RESUMEN

The strength of synaptic transmission between a neuron and multiple postsynaptic partners can vary considerably. We have studied synaptic heterogeneity using the glutamatergic Drosophila neuromuscular junction (NMJ), which contains multiple synaptic connections of varying strengths between a motor axon and muscle fiber. In larval NMJs, there is a gradient of synaptic transmission from weak proximal to strong distal boutons. We imaged synaptic transmission with the postsynaptically targeted fluorescent calcium sensor SynapCam, to investigate the molecular pathways that determine synaptic strength and set up this gradient. We discovered that mutations in the Bone Morphogenetic Protein (BMP) signaling pathway disrupt production of strong distal boutons. We find that strong connections contain unbundled microtubules in the boutons, suggesting a role for microtubule organization in transmission strength. The spastin mutation, which disorganizes microtubules, disrupted the transmission gradient, supporting this interpretation. We propose that the BMP pathway, shown previously to function in the homeostatic regulation of synaptic growth, also boosts synaptic transmission in a spatially selective manner that depends on the microtubule system.

Asunto(s)

Proteínas Morfogenéticas Óseas/metabolismo; Microtúbulos/metabolismo; Unión Neuromuscular/fisiología; Terminales Presinápticos/fisiología; Transmisión Sináptica/fisiología; Adenosina Trifosfatasas/genética; Adenosina Trifosfatasas/metabolismo; Animales; Animales Modificados Genéticamente; Proteínas Morfogenéticas Óseas/genética; Calcio/metabolismo; Proteínas de Unión al ADN/genética; Proteínas de Unión al ADN/metabolismo; Drosophila; Proteínas de Drosophila/genética; Proteínas de Drosophila/metabolismo; Femenino; Colorantes Fluorescentes; Inmunohistoquímica; Proteínas Asociadas a Microtúbulos/genética; Proteínas Asociadas a Microtúbulos/metabolismo; Técnicas de Placa-Clamp; Receptores de Superficie Celular/genética; Receptores de Superficie Celular/metabolismo; Factores de Transcripción/genética; Factores de Transcripción/metabolismo

Palabras clave

Bone Morphogenetic Protein signaling pathway; drosophila; fluorescent calcium sensor; microtubule; neuromuscular junction; synaptic transmission

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Terminales Presinápticos / Transmisión Sináptica / Proteínas Morfogenéticas Óseas / Microtúbulos / Unión Neuromuscular Límite: Animals Idioma: En Revista: Neuroscience Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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