Your browser doesn't support javascript.
loading
Nanogel-based pneumococcal surface protein A nasal vaccine induces microRNA-associated Th17 cell responses with neutralizing antibodies against Streptococcus pneumoniae in macaques.
Fukuyama, Y; Yuki, Y; Katakai, Y; Harada, N; Takahashi, H; Takeda, S; Mejima, M; Joo, S; Kurokawa, S; Sawada, S; Shibata, H; Park, E J; Fujihashi, K; Briles, D E; Yasutomi, Y; Tsukada, H; Akiyoshi, K; Kiyono, H.
Afiliación
  • Fukuyama Y; Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
  • Yuki Y; 1] Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan [2] International Research and Development Center for Mucosal Vaccine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
  • Katakai Y; Corporation for Production and Research of Laboratory Primates, Tsukuba, Ibaraki, Japan.
  • Harada N; PET Center, Central Research Laboratory, Hamamatsu Photonics K.K., Hamamatsu, Shizuoka, Japan.
  • Takahashi H; Department of Polymer Chemistry, Kyoto University Graduate School of Engineering, Nishikyo-ku, Kyoto, Japan.
  • Takeda S; Department of Polymer Chemistry, Kyoto University Graduate School of Engineering, Nishikyo-ku, Kyoto, Japan.
  • Mejima M; Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
  • Joo S; Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
  • Kurokawa S; Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
  • Sawada S; Department of Polymer Chemistry, Kyoto University Graduate School of Engineering, Nishikyo-ku, Kyoto, Japan.
  • Shibata H; Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Tsukuba, Ibaraki, Japan.
  • Park EJ; Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
  • Fujihashi K; Departments of Pediatric Dentistry and Microbiology, The Immunobiology Vaccine Center, The University of Alabama at Birmingham, Birmingham, Alabama, USA.
  • Briles DE; Department of Microbiology, The University of Alabama at Birmingham, Birmingham, Alabama, USA.
  • Yasutomi Y; Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Tsukuba, Ibaraki, Japan.
  • Tsukada H; PET Center, Central Research Laboratory, Hamamatsu Photonics K.K., Hamamatsu, Shizuoka, Japan.
  • Akiyoshi K; Department of Polymer Chemistry, Kyoto University Graduate School of Engineering, Nishikyo-ku, Kyoto, Japan.
  • Kiyono H; 1] Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan [2] International Research and Development Center for Mucosal Vaccine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
Mucosal Immunol ; 8(5): 1144-53, 2015 Sep.
Article en En | MEDLINE | ID: mdl-25669148
We previously established a nanosized nasal vaccine delivery system by using a cationic cholesteryl group-bearing pullulan nanogel (cCHP nanogel), which is a universal protein-based antigen-delivery vehicle for adjuvant-free nasal vaccination. In the present study, we examined the central nervous system safety and efficacy of nasal vaccination with our developed cCHP nanogel containing pneumococcal surface protein A (PspA-nanogel) against pneumococcal infection in nonhuman primates. When [(18)F]-labeled PspA-nanogel was nasally administered to a rhesus macaque (Macaca mulatta), longer-term retention of PspA was noted in the nasal cavity when compared with administration of PspA alone. Of importance, no deposition of [(18)F]-PspA was seen in the olfactory bulbs or brain. Nasal PspA-nanogel vaccination effectively induced PspA-specific serum IgG with protective activity and mucosal secretory IgA (SIgA) Ab responses in cynomolgus macaques (Macaca fascicularis). Nasal PspA-nanogel-induced immune responses were mediated through T-helper (Th) 2 and Th17 cytokine responses concomitantly with marked increases in the levels of miR-181a and miR-326 in the serum and respiratory tract tissues, respectively, of the macaques. These results demonstrate that nasal PspA-nanogel vaccination is a safe and effective strategy for the development of a nasal vaccine for the prevention of pneumonia in humans.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptococcus pneumoniae / Proteínas Bacterianas / Portadores de Fármacos / MicroARNs / Nanopartículas / Anticuerpos Neutralizantes / Glucanos / Anticuerpos Antibacterianos Tipo de estudio: Risk_factors_studies Límite: Animals / Female / Humans / Male Idioma: En Revista: Mucosal Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptococcus pneumoniae / Proteínas Bacterianas / Portadores de Fármacos / MicroARNs / Nanopartículas / Anticuerpos Neutralizantes / Glucanos / Anticuerpos Antibacterianos Tipo de estudio: Risk_factors_studies Límite: Animals / Female / Humans / Male Idioma: En Revista: Mucosal Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos