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In vitro activity of five quinolones and analysis of the quinolone resistance-determining regions of gyrA, gyrB, parC, and parE in Ureaplasma parvum and Ureaplasma urealyticum clinical isolates from perinatal patients in Japan.
Kawai, Yasuhiro; Nakura, Yukiko; Wakimoto, Tetsu; Nomiyama, Makoto; Tokuda, Tsugumichi; Takayanagi, Toshimitsu; Shiraishi, Jun; Wasada, Kenshi; Kitajima, Hiroyuki; Fujita, Tomio; Nakayama, Masahiro; Mitsuda, Nobuaki; Nakanishi, Isao; Takeuchi, Makoto; Yanagihara, Itaru.
Afiliación
  • Kawai Y; Department of Developmental Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka, Japan.
  • Nakura Y; Department of Developmental Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka, Japan.
  • Wakimoto T; Department of Developmental Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka, Japan Department of Obstetrics and Gynecology, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
  • Nomiyama M; Department of Obstetrics and Gynecology, Perinatal Center, National Hospital Organization Saga National Hospital, Saga City, Saga, Japan.
  • Tokuda T; Department of Obstetrics and Gynecology, Perinatal Center, National Hospital Organization Saga National Hospital, Saga City, Saga, Japan.
  • Takayanagi T; Department of Pediatrics, National Hospital Organization Saga National Hospital, Saga City, Saga, Japan.
  • Shiraishi J; Department of Neonatology, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka, Japan.
  • Wasada K; Department of Obstetrics and Gynecology, Aizenbashi Hospital, Osaka City, Osaka, Japan.
  • Kitajima H; Department of Neonatology, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka, Japan.
  • Fujita T; Fujita Clinic, Osaka City, Osaka, Japan.
  • Nakayama M; Department of Pathology and Laboratory Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka, Japan.
  • Mitsuda N; Department of Obstetrics, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka, Japan.
  • Nakanishi I; Department of Maternal Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka, Japan.
  • Takeuchi M; Department of Pathology and Laboratory Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka, Japan.
  • Yanagihara I; Department of Developmental Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka, Japan itaruy@mch.pref.osaka.jp.
Antimicrob Agents Chemother ; 59(4): 2358-64, 2015 Apr.
Article en En | MEDLINE | ID: mdl-25645833
Ureaplasma spp. cause several disorders, such as nongonococcal urethritis, miscarriage, and preterm delivery with lung infections in neonates, characterized by pathological chorioamnionitis in the placenta. Although reports on antibiotic resistance in Ureaplasma are on the rise, reports on quinolone-resistant Ureaplasma infections in Japan are limited. The purpose of this study was to determine susceptibilities to five quinolones of Ureaplasma urealyticum and Ureaplasma parvum isolated from perinatal samples in Japan and to characterize the quinolone resistance-determining regions in the gyrA, gyrB, parC, and parE genes. Out of 28 clinical Ureaplasma strains, we isolated 9 with high MICs of quinolones and found a single parC gene mutation, resulting in the change S83L. Among 158 samples, the ParC S83L mutation was found in 37 samples (23.4%), including 1 sample harboring a ParC S83L-GyrB P462S double mutant. Novel mutations of ureaplasmal ParC (S83W and S84P) were independently found in one of the samples. Homology modeling of the ParC S83W mutant suggested steric hindrance of the quinolone-binding pocket (QBP), and de novo prediction of peptide structures revealed that the ParC S84P may break/kink the formation of the α4 helix in the QBP. Further investigations are required to unravel the extent and mechanism of antibiotic resistance of Ureaplasma spp. in Japan.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ureaplasma / Ureaplasma urealyticum / Infecciones por Ureaplasma / Quinolonas / Girasa de ADN / Topoisomerasa de ADN IV / Farmacorresistencia Bacteriana Tipo de estudio: Prognostic_studies Límite: Adult / Female / Humans / Pregnancy País/Región como asunto: Asia Idioma: En Revista: Antimicrob Agents Chemother Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ureaplasma / Ureaplasma urealyticum / Infecciones por Ureaplasma / Quinolonas / Girasa de ADN / Topoisomerasa de ADN IV / Farmacorresistencia Bacteriana Tipo de estudio: Prognostic_studies Límite: Adult / Female / Humans / Pregnancy País/Región como asunto: Asia Idioma: En Revista: Antimicrob Agents Chemother Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos