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A comparison of physiological and transcriptome responses to water deprivation and salt loading in the rat supraoptic nucleus.
Greenwood, Michael P; Mecawi, Andre S; Hoe, See Ziau; Mustafa, Mohd Rais; Johnson, Kory R; Al-Mahmoud, Ghada A; Elias, Lucila L K; Paton, Julian F R; Antunes-Rodrigues, Jose; Gainer, Harold; Murphy, David; Hindmarch, Charles C T.
Afiliación
  • Greenwood MP; School of Clinical Sciences, University of Bristol, Bristol, United Kingdom;
  • Mecawi AS; Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Physiological Sciences, Institute of Biology, Federal Rural University of Rio de Janeiro, Seropedica, Brazil;
  • Hoe SZ; Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;
  • Mustafa MR; Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;
  • Johnson KR; Clinical Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland;
  • Al-Mahmoud GA; Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, Al Tarfa, Doha, Qatar;
  • Elias LL; Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil;
  • Paton JF; School of Physiology and Pharmacology, University Walk, Bristol, United Kingdom; and.
  • Antunes-Rodrigues J; Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil;
  • Gainer H; Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland.
  • Murphy D; School of Clinical Sciences, University of Bristol, Bristol, United Kingdom; Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;
  • Hindmarch CC; School of Clinical Sciences, University of Bristol, Bristol, United Kingdom; Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; c.hindmarch@bristol.ac.uk.
Am J Physiol Regul Integr Comp Physiol ; 308(7): R559-68, 2015 Apr 01.
Article en En | MEDLINE | ID: mdl-25632023
Salt loading (SL) and water deprivation (WD) are experimental challenges that are often used to study the osmotic circuitry of the brain. Central to this circuit is the supraoptic nucleus (SON) of the hypothalamus, which is responsible for the biosynthesis of the hormones, arginine vasopressin (AVP) and oxytocin (OXT), and their transport to terminals that reside in the posterior lobe of the pituitary. On osmotic challenge evoked by a change in blood volume or osmolality, the SON undergoes a function-related plasticity that creates an environment that allows for an appropriate hormone response. Here, we have described the impact of SL and WD compared with euhydrated (EU) controls in terms of drinking and eating behavior, body weight, and recorded physiological data including circulating hormone data and plasma and urine osmolality. We have also used microarrays to profile the transcriptome of the SON following SL and remined data from the SON that describes the transcriptome response to WD. From a list of 2,783 commonly regulated transcripts, we selected 20 genes for validation by qPCR. All of the 9 genes that have already been described as expressed or regulated in the SON by osmotic stimuli were confirmed in our models. Of the 11 novel genes, 5 were successfully validated while 6 were false discoveries.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Supraóptico / Privación de Agua / Cloruro de Sodio Dietético / Transcriptoma Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Am J Physiol Regul Integr Comp Physiol Asunto de la revista: FISIOLOGIA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Supraóptico / Privación de Agua / Cloruro de Sodio Dietético / Transcriptoma Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Am J Physiol Regul Integr Comp Physiol Asunto de la revista: FISIOLOGIA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos