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Biomarkers of brevetoxin exposure and composite toxin levels in hard clam (Mercenaria sp.) exposed to Karenia brevis blooms.
Abraham, Ann; El Said, Kathleen R; Wang, Yuesong; Jester, Edward L E; Plakas, Steven M; Flewelling, Leanne J; Henry, Michael S; Pierce, Richard H.
Afiliación
  • Abraham A; FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, 1 Iberville Drive, Dauphin Island, AL 36528, USA. Electronic address: ann.abraham@fda.hhs.gov.
  • El Said KR; FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, 1 Iberville Drive, Dauphin Island, AL 36528, USA.
  • Wang Y; FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, 1 Iberville Drive, Dauphin Island, AL 36528, USA.
  • Jester EL; FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, 1 Iberville Drive, Dauphin Island, AL 36528, USA.
  • Plakas SM; FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, 1 Iberville Drive, Dauphin Island, AL 36528, USA.
  • Flewelling LJ; Fish and Wildlife Research Institute, 100 Eighth Avenue SE, St. Petersburg, FL 33701, USA.
  • Henry MS; Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL 34236, USA.
  • Pierce RH; Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL 34236, USA.
Toxicon ; 96: 82-8, 2015 Mar.
Article en En | MEDLINE | ID: mdl-25620222
Brevetoxins in clams (Mercenaria sp.) exposed to recurring blooms of Karenia brevis in Sarasota Bay, FL, were studied over a three-year period. Brevetoxin profiles in toxic clams were generated by ELISA and LC-MS. Several brevetoxin metabolites, as identified by LC-MS, were major contributors to the composite brevetoxin response of ELISA. These were S-desoxyBTX-B2 (m/z 1018), BTX-B2 (m/z 1034), BTX-B5 (m/z 911), open A-ring BTX-B5 (m/z 929), and BTX-B1 (m/z 1018). Summed values of these metabolites were highly correlated (R(2) = 0.9) with composite B-type brevetoxin measurements by ELISA. S-desoxyBTX-B2, BTX-B2, and BTX-B1 were the most persistent and detectable in shellfish for several months after dissipation of blooms. These metabolites were selected as LC-MS biomarkers of brevetoxin exposure and reflective of composite B-type brevetoxins in hard clam. ELISA and LC-MS values were moderately correlated with toxicity of the shellfish by mouse bioassay. ELISA and LC-MS methods offer rapid screening and confirmatory determination of brevetoxins, respectively, as well as toxicity assessment in clams exposed to K. brevis blooms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dinoflagelados / Biomarcadores / Bivalvos / Oxocinas / Exposición a Riesgos Ambientales / Floraciones de Algas Nocivas / Toxinas Marinas Tipo de estudio: Prognostic_studies Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: Toxicon Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dinoflagelados / Biomarcadores / Bivalvos / Oxocinas / Exposición a Riesgos Ambientales / Floraciones de Algas Nocivas / Toxinas Marinas Tipo de estudio: Prognostic_studies Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: Toxicon Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido