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The cryptococcal antigen lateral flow assay: A point-of-care diagnostic at an opportune time.
Tang, Michele W; Clemons, Karl V; Katzenstein, David A; Stevens, David A.
Afiliación
  • Tang MW; a The Division of Infectious Diseases and Geographic Medicine , Stanford University , Stanford , CA , USA and.
  • Clemons KV; b California Institute for Medical Research , San Jose , CA , USA.
  • Katzenstein DA; a The Division of Infectious Diseases and Geographic Medicine , Stanford University , Stanford , CA , USA and.
  • Stevens DA; b California Institute for Medical Research , San Jose , CA , USA.
Crit Rev Microbiol ; 42(4): 634-42, 2016 Aug.
Article en En | MEDLINE | ID: mdl-25612826
Cryptococcal meningitis is a devastating HIV-related opportunistic infection, affecting nearly 1 million individuals and causing over 500 000 deaths each year. The burden of disease is greatest in sub-Saharan Africa and Southeast Asia, where cryptococcal disease is the most common cause of meningitis. Rapid, accurate and affordable diagnosis of cryptococcal disease has been lacking in many of the most heavily affected areas. Here, we review a point-of-care assay for cryptococcal disease, the dipstick-formatted cryptococcal antigen lateral flow assay (LFA) (IMMY, Norman, OK). In comparison to culture, the assay is 99.5% sensitive and 98% specific. In comparison to other commercially available tests for cryptococcal antigen, the LFA has equal or superior sensitivity and specificity in CSF, plasma and serum samples. We discuss potential applications for the use of the assay in resource-limited settings, including what is likely to be an important role of the LFA in screening for early cryptococcal infection before clinical disease and in evaluating pre-emptive treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía de Afinidad / Meningitis Criptocócica / Sistemas de Atención de Punto / Pruebas Diagnósticas de Rutina / Antígenos Fúngicos Tipo de estudio: Diagnostic_studies Idioma: En Revista: Crit Rev Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía de Afinidad / Meningitis Criptocócica / Sistemas de Atención de Punto / Pruebas Diagnósticas de Rutina / Antígenos Fúngicos Tipo de estudio: Diagnostic_studies Idioma: En Revista: Crit Rev Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido