Characterization of co-purified acellular pertussis vaccines.

Xu, Yinghua; Tan, Yajun; Asokanathan, Catpagavalli; Zhang, Shumin; Xing, Dorothy; Wang, Junzhi

Xu, Yinghua; Tan, Yajun; Asokanathan, Catpagavalli; Zhang, Shumin; Xing, Dorothy; Wang, Junzhi.

Afiliación

Xu Y; a Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products ; National Institutes for Food and Drug Control ; Beijing , PR China ;

Hum Vaccin Immunother ; 11(2): 421-7, 2015.

Article en En | MEDLINE | ID: mdl-25610957

RESUMEN

Whole-cell pertussis vaccines (WPVs) have been completely replaced by the co-purified acellular vaccines (APVs) in China. To date few laboratory studies were reported for co-purified APVs in terms of their antigenic composition and protective immune responses. To further understand the antigenic composition in co-purified APVs, in the present study 2-dimensional gel electrophoresis-based proteomic technology was used to analyze the composition of co-purified APVs. The results showed that besides the main antigens pertussis toxin (PT) and filamentous hemagglutinin (FHA), co-purified APVs also contained pertactin (PRN), fimbriae (FIM) 2and3 and other minor protein antigens. Of the 9 proteins identified, 3 were differentially presented in products from manufacturer 1 and manufacturer 2. Compared with WPVs and purified APVs, co-purified APVs induced a mixed Th1/Th2 immune response with more toward to a Th1 response than the purified APVs in this study. These results hint that different immune mechanisms might be involved in protection induced by co-purified and purified APVs.

Asunto(s)

Antígenos Bacterianos/análisis; Vacuna contra la Tos Ferina/química; Vacuna contra la Tos Ferina/aislamiento & purificación; Tos Ferina/prevención & control; Animales; Anticuerpos Antibacterianos/sangre; China; Electroforesis en Gel Bidimensional; Femenino; Ratones; Vacuna contra la Tos Ferina/administración & dosificación; Vacuna contra la Tos Ferina/inmunología; Proteoma/análisis; Células TH1/inmunología; Células Th2/inmunología; Vacunas Acelulares/administración & dosificación; Vacunas Acelulares/química; Vacunas Acelulares/inmunología; Vacunas Acelulares/aislamiento & purificación

Palabras clave

2-DE, 2-dimensional gel electrophoresis; APVs, acellular pertussis vaccines; BipA, putative outer membrane ligand binding protein; Co-purified acellular pertussis vaccines; FHA, filamentous hemagglutinin; FIM, fimbriae; PRN, pertactin; PT, pertussis toxin; SHD, single human dose; Sbp, sulfate-binding protein precursor; WPVs, whole-cell pertussis vaccines; bvg, Bordetella virulence regulon; immune responses; protection; proteomic analysis; sphB1, autotransporter subtilisin-like protease

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacuna contra la Tos Ferina / Tos Ferina / Antígenos Bacterianos Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Hum Vaccin Immunother Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos

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