[Effects on expression of osteogenesisgene in the osteoblast with endoplasmic reticulum stress induced by fluoride].
Wei Sheng Yan Jiu
; 43(6): 967-71, 2014 Nov.
Article
en Zh
| MEDLINE
| ID: mdl-25603608
OBJECTIVE: To explore the gene expressions of endoplasmic reticulum stress and differentiation in osteoblast treated by excess fluoride. METHODS: Using primary cultured human osteoblasts for fluorosis model in vitro, apoptosis was inspected by flow cytometer, and RNA was extracted for examination of the unfolded protein response and bone differentiation genes. RESULTS: Fluoride could cause endoplasm reticulum stress in osteoblasts by 15 genes upregulated, 1 gene downregulated. These genes involved PERK, IRE1 and ATF6 signaling pathways of endoplasmic reticulum stress. Meanwhile 32 osteogenesis genes were upregulated, and 2 genes downregulated, involving collagen, matrix metalloproteinase, integrin, bone morphogenetic protein, vascular endothelial growth factor, and tumor necrosis factor gene. CONCLUSION: Excess fluoride can cause endoplasmic stress in osteoblast, while have an impact on the gene expression of osteogenesis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Osteogénesis
/
Respuesta de Proteína Desplegada
/
Estrés del Retículo Endoplásmico
/
Fluoruros
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Wei Sheng Yan Jiu
Asunto de la revista:
SAUDE PUBLICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China