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Glutathionylspermidine in the modification of protein SH groups: the enzymology and its application to study protein glutathionylation.
Lin, Jason Ching-Yao; Chiang, Bing-Yu; Chou, Chi-Chi; Chen, Tzu-Chieh; Chen, Yi-Ju; Chen, Yu-Ju; Lin, Chun-Hung.
Afiliación
  • Lin JC; Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529, Taiwan. jcl@gate.sinica.edu.tw.
  • Chiang BY; Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529, Taiwan. bingyuchiang@gmail.com.
  • Chou CC; Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529, Taiwan. sandra77@gate.sinica.edu.tw.
  • Chen TC; Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529, Taiwan. ebioche@gmail.com.
  • Chen YJ; Institute of Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529, Taiwan. yjchen@chem.sinica.edu.tw.
  • Chen YJ; Institute of Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529, Taiwan. yjchen@chem.sinica.edu.tw.
  • Lin CH; Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529, Taiwan. chunhung@gate.sinica.edu.tw.
Molecules ; 20(1): 1452-74, 2015 Jan 15.
Article en En | MEDLINE | ID: mdl-25599150
Cysteine is very susceptible to reactive oxygen species. In response; posttranslational thiol modifications such as reversible disulfide bond formation have arisen as protective mechanisms against undesired in vivo cysteine oxidation. In Gram-negative bacteria a major defense mechanism against cysteine overoxidation is the formation of mixed protein disulfides with low molecular weight thiols such as glutathione and glutathionylspermidine. In this review we discuss some of the mechanistic aspects of glutathionylspermidine in prokaryotes and extend its potential use to eukaryotes in proteomics and biochemical applications through an example with tissue transglutaminase and its S-glutathionylation.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Sulfhidrilo / Proteínas / Espermidina / Procesamiento Proteico-Postraduccional / Glutatión Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Sulfhidrilo / Proteínas / Espermidina / Procesamiento Proteico-Postraduccional / Glutatión Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza