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Proteomic analysis of urethral protein expression in an estrogen receptor α-deficient murine model of stress urinary incontinence.
Chen, Yung-Hsiang; Chen, Chao-Jung; Lin, Yu-Ning; Wu, Yang-Chang; Hsieh, Wen-Tsong; Wu, Bor-Tsang; Ma, Wen-Lung; Chen, Wen-Chi; Tsai, Kao-Sung; Wu, San-Yuan; Chang, Chawnshang; Chen, Huey-Yi; Yeh, Shuyuan.
Afiliación
  • Chen YH; Graduate Institute of Integrated Medicine, School of Chinese Medicine, College of Chinese Medicine, School of Pharmacy, College of Pharmacy, Department of Pharmacology, Department of Physical Therapy, Graduate Institute of Rehabilitation Science, Research Center for Chinese Medicine and Acupuncture,
  • Chen CJ; Departments of Medical Research, Urology, and Obstetrics and Gynecology, Proteomics Core Laboratory, Sex Hormone Research Center, Chinese Medicine Research and Development Center, Center for Molecular Medicine, China Medical University Hospital, Taichung, 40402, Taiwan. d87a03@ym.edu.tw.
  • Lin YN; Department of Psychology, College of Medical and Health Science, Asia University, Taichung, 41354, Taiwan. d87a03@ym.edu.tw.
  • Wu YC; Graduate Institute of Integrated Medicine, School of Chinese Medicine, College of Chinese Medicine, School of Pharmacy, College of Pharmacy, Department of Pharmacology, Department of Physical Therapy, Graduate Institute of Rehabilitation Science, Research Center for Chinese Medicine and Acupuncture,
  • Hsieh WT; Departments of Medical Research, Urology, and Obstetrics and Gynecology, Proteomics Core Laboratory, Sex Hormone Research Center, Chinese Medicine Research and Development Center, Center for Molecular Medicine, China Medical University Hospital, Taichung, 40402, Taiwan.
  • Wu BT; Graduate Institute of Integrated Medicine, School of Chinese Medicine, College of Chinese Medicine, School of Pharmacy, College of Pharmacy, Department of Pharmacology, Department of Physical Therapy, Graduate Institute of Rehabilitation Science, Research Center for Chinese Medicine and Acupuncture,
  • Ma WL; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.
  • Chen WC; Graduate Institute of Integrated Medicine, School of Chinese Medicine, College of Chinese Medicine, School of Pharmacy, College of Pharmacy, Department of Pharmacology, Department of Physical Therapy, Graduate Institute of Rehabilitation Science, Research Center for Chinese Medicine and Acupuncture,
  • Tsai KS; Graduate Institute of Integrated Medicine, School of Chinese Medicine, College of Chinese Medicine, School of Pharmacy, College of Pharmacy, Department of Pharmacology, Department of Physical Therapy, Graduate Institute of Rehabilitation Science, Research Center for Chinese Medicine and Acupuncture,
  • Wu SY; Departments of Medical Research, Urology, and Obstetrics and Gynecology, Proteomics Core Laboratory, Sex Hormone Research Center, Chinese Medicine Research and Development Center, Center for Molecular Medicine, China Medical University Hospital, Taichung, 40402, Taiwan.
  • Chang C; Graduate Institute of Integrated Medicine, School of Chinese Medicine, College of Chinese Medicine, School of Pharmacy, College of Pharmacy, Department of Pharmacology, Department of Physical Therapy, Graduate Institute of Rehabilitation Science, Research Center for Chinese Medicine and Acupuncture,
  • Chen HY; Departments of Medical Research, Urology, and Obstetrics and Gynecology, Proteomics Core Laboratory, Sex Hormone Research Center, Chinese Medicine Research and Development Center, Center for Molecular Medicine, China Medical University Hospital, Taichung, 40402, Taiwan.
  • Yeh S; Graduate Institute of Integrated Medicine, School of Chinese Medicine, College of Chinese Medicine, School of Pharmacy, College of Pharmacy, Department of Pharmacology, Department of Physical Therapy, Graduate Institute of Rehabilitation Science, Research Center for Chinese Medicine and Acupuncture,
World J Urol ; 33(10): 1635-43, 2015 Oct.
Article en En | MEDLINE | ID: mdl-25577129
PURPOSE: The roles of estrogen receptor α (ERα) in stress urinary incontinence (SUI) remain elusive. This study was conducted to understand the molecular mechanism of ERα against SUI. METHODS: Wild-type (ERα(+/+)) and ACTB-cre ERα knockout (ERα(-/-)) female mice were generated. Urethral function and protein expression were measured. Leak point pressures (LPP) and maximum urethral closure pressure (MUCP) were assessed in mice under urethane anesthesia. After the measurements, the urethras were removed for proteomic analysis using the two-dimensional differential gel electrophoresis and liquid chromatography-mass spectrometry technology. Interaction between these ERα pathway proteins was further analyzed by using MetaCore. Lastly, Western blot and immunochemistry (IHC) were used to confirm the candidate protein expression levels and locations, respectively. RESULTS: Compared with the ERα(+/+) group, the LPP and MUCP values of the ERα(-/-) group were significantly decreased. Additionally, we identified 11 differentially expressed proteins in the urethra of ERα(-/-) female mice; five proteins were down-regulated and six were up-regulated. The majority of the ERα knockout-modified proteins were involved in muscle development, contraction, and regulation, as well as immune response (amphoterin signaling and phagocytosis), proteolysis, and cell adhesion (platelet aggregation and integrin-mediated cell-matrix adhesion). IHC and Western blot confirmed the down-regulation of tropomyosin and up-regulation of myosin in urethra. CONCLUSIONS: This is the first study to estimate protein expression changes in urethras from ERα(-/-) female mice. These changes could be related to the molecular mechanism of ERα in SUI.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Uretra / Incontinencia Urinaria de Esfuerzo / ARN / Regulación de la Expresión Génica / Proteómica / Receptor alfa de Estrógeno Límite: Animals Idioma: En Revista: World J Urol Año: 2015 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Uretra / Incontinencia Urinaria de Esfuerzo / ARN / Regulación de la Expresión Génica / Proteómica / Receptor alfa de Estrógeno Límite: Animals Idioma: En Revista: World J Urol Año: 2015 Tipo del documento: Article Pais de publicación: Alemania