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Identification of crotamine in the venom of Crotalus durissus collilineatus by three different methods.
de Oliveira, Sayonara Ay More; Magalhães, Marta Regina; Salazar, Vania Cristina Rodríguez; Valadares, Marize Campos; da Cunha, Luiz Carlos.
Afiliación
  • de Oliveira SA; Núcleo de Estudos e Pesquisas Tóxico-Farmacológicas, Faculdade de Farmácia, Universidade Federal de Goiás, 74.605-220 Goiânia, GO, Brazil; Centro de Estudos e Pesquisas Biológicas, Departamento de Biologia, Pontifícia Universidade Católica de Goiás, 74.605-010 Goiânia, GO, Brazil. Electronic address
  • Magalhães MR; Centro de Estudos e Pesquisas Biológicas, Departamento de Biologia, Pontifícia Universidade Católica de Goiás, 74.605-010 Goiânia, GO, Brazil.
  • Salazar VC; Núcleo de Estudos e Pesquisas Tóxico-Farmacológicas, Faculdade de Farmácia, Universidade Federal de Goiás, 74.605-220 Goiânia, GO, Brazil.
  • Valadares MC; Laboratório de Farmacologia e Toxicologia Celular, Faculdade de Farmácia, Universidade Federal de Goiás, 74.605-010 Goiânia, GO, Brazil.
  • da Cunha LC; Núcleo de Estudos e Pesquisas Tóxico-Farmacológicas, Faculdade de Farmácia, Universidade Federal de Goiás, 74.605-220 Goiânia, GO, Brazil.
Toxicon ; 95: 46-51, 2015 Mar.
Article en En | MEDLINE | ID: mdl-25553594
The use of mice for the identification of crotamine has been the motive of discussions of bioethical character and technical (efficiency), so that a reassessment of the use of animals in experiments is global trend the search for alternative tests. The objective of this study was to standardize a method for HPLC-PDA to identify the presence of crotamine in the venom of rattlesnakes, aiming to propose an alternative methodology to reduce or replace the use of animals. The Cdc was evaluated as to the presence of crotamine by 3 methods: traditional test lethality in mice (Mus musculus) swiss albino male, 18-22 g (i.p.), polyacrylamide gel electrophoresis (SDS-PAGE) and HPLC-PDA. The venoms of 50 specimens of Crotalus durissus collilineatus, held in the serpentarium CEPB/PUC Goiás, were obtained by manual massage of the gland, making the collection individually. To identify the band corresponding to crotamine, the venoms of specimens, analysis was performed on SDS-PAGE and references. Procedure in mice with 20% of the samples tested positive for crotamine, 24% negative and 56% uncertain outcome. With the SDS-PAGE was identified crotamine in 26% of samples, 26% negative and 48% continued with uncertain outcome. By HPLC method showed the presence myotoxin in 86% of samples, with 14% negative. The tests conducted in this study indicated that methodology which utilizes animals for identifying the presence of crotamine the venom of C. durissus can safely be replaced by the test SDS-PAGE and HPLC, since the methods are reproducible, and do not undergo any interference biological animal and mainly contribute to reducing the number of animals used for laboratory tests.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Crotalus / Venenos de Crotálidos / Alternativas al Uso de Animales Tipo de estudio: Diagnostic_studies / Prognostic_studies Aspecto: Ethics Límite: Animals Idioma: En Revista: Toxicon Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Crotalus / Venenos de Crotálidos / Alternativas al Uso de Animales Tipo de estudio: Diagnostic_studies / Prognostic_studies Aspecto: Ethics Límite: Animals Idioma: En Revista: Toxicon Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido