Label-free evanescent microscopy for membrane nano-tomography in living cells.
J Biophotonics
; 7(11-12): 857-62, 2014 Nov.
Article
en En
| MEDLINE
| ID: mdl-25538972
We show that through-the-objective evanescent microscopy (epi-EM) is a powerful technique to image membranes in living cells. Readily implementable on a standard inverted microscope, this technique enables full-field and real-time tracking of membrane processes without labeling and thus signal fading. In addition, we demonstrate that the membrane/interface distance can be retrieved with 10 nm precision using a multilayer Fresnel model. We apply this nano-axial tomography of living cell membranes to retrieve quantitative information on membrane invagination dynamics.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tomografía Computarizada por Rayos X
/
Nanotecnología
/
Microscopía Fluorescente
Límite:
Humans
Idioma:
En
Revista:
J Biophotonics
Asunto de la revista:
BIOFISICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Alemania