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An RNA binding protein promotes axonal integrity in peripheral neurons by destabilizing REST.
Cargnin, Francesca; Nechiporuk, Tamilla; Müllendorff, Karin; Stumpo, Deborah J; Blackshear, Perry J; Ballas, Nurit; Mandel, Gail.
Afiliación
  • Cargnin F; Howard Hughes Medical Institute, Vollum Institute, Oregon Health & Science University, Portland, Oregon 97239.
  • Nechiporuk T; Howard Hughes Medical Institute, Vollum Institute, Oregon Health & Science University, Portland, Oregon 97239.
  • Müllendorff K; Howard Hughes Medical Institute, Vollum Institute, Oregon Health & Science University, Portland, Oregon 97239.
  • Stumpo DJ; Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, and.
  • Blackshear PJ; Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, and.
  • Ballas N; Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794.
  • Mandel G; Howard Hughes Medical Institute, Vollum Institute, Oregon Health & Science University, Portland, Oregon 97239, mandelg@ohsu.edu.
J Neurosci ; 34(50): 16650-61, 2014 Dec 10.
Article en En | MEDLINE | ID: mdl-25505318
The RE1 Silencing Transcription Factor (REST) acts as a governor of the mature neuronal phenotype by repressing a large consortium of neuronal genes in non-neuronal cells. In the developing nervous system, REST is present in progenitors and downregulated at terminal differentiation to promote acquisition of mature neuronal phenotypes. Paradoxically, REST is still detected in some regions of the adult nervous system, but how REST levels are regulated, and whether REST can still repress neuronal genes, is not known. Here, we report that homeostatic levels of REST are maintained in mature peripheral neurons by a constitutive post-transcriptional mechanism. Specifically, using a three-hybrid genetic screen, we identify the RNA binding protein, ZFP36L2, associated previously only with female fertility and hematopoiesis, and show that it regulates REST mRNA stability. Dorsal root ganglia in Zfp36l2 knock-out mice, or wild-type ganglia expressing ZFP36L2 shRNA, show higher steady-state levels of Rest mRNA and protein, and extend thin and disintegrating axons. This phenotype is due, at least in part, to abnormally elevated REST levels in the ganglia because the axonal phenotype is attenuated by acute knockdown of REST in Zfp36l2 KO DRG explants. The higher REST levels result in lower levels of target genes, indicating that REST can still fine-tune gene expression through repression. Thus, REST levels are titrated in mature peripheral neurons, in part through a ZFP36L2-mediated post-transcriptional mechanism, with consequences for axonal integrity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Represoras / Axones / Proteínas de Unión al ARN / Tristetraprolina / Ganglios Espinales Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Neurosci Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Represoras / Axones / Proteínas de Unión al ARN / Tristetraprolina / Ganglios Espinales Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Neurosci Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos