Au nanoparticle/graphene nanocomposite as a platform for the sensitive detection of NADH in human urine.
Biosens Bioelectron
; 66: 474-80, 2015 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-25499660
Here we report on a facile, rapid, sensitive, selective and highly stable electrochemical sensing platform for ß-nicotinamide adenine dinucleotide (NADH) based on uncapped Au nanoparticle/reduced graphene oxide (rGO) nanocomposites without the aid of any redox mediators and enzymes. The Au nanoparticle/rGO composite sensing platform was directly formed on a glassy carbon electrode through an in situ electrochemical reduction of GO and Au(3+) with a 100% usage of the precursors. The as-prepared Au nanoparticle/rGO composites demonstrated excellent direct electrocatalytic oxidation toward NADH, providing a large electrochemical active surface area as well as a favorable environment for electron transfer from NADH to the electrode via the enhanced mobility of charge carriers. The Au nanoparticle/rGO composites offered a ~2.3 times higher electrocatalytic current density with a negative shift of 112mV, in comparison to Au nanoparticles. The sensor developed in this study displayed a high sensitivity of 0.916µA/µMcm(2) and a wide linear range of from 50nM to 500µM with a limit of detection of 1.13nM (S/N=3). The interferences from the common interferents such as glutathione, glucose, ascorbic acid and quanine were negligible. The prepared sensor was further tested for the determination of NADH in human urine samples, showing the Au nanoparticle/rGO nanocomposites simultaneously formed by one-step electrochemical reduction have promising biomedical applications.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
NAD
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2015
Tipo del documento:
Article
Pais de publicación:
Reino Unido