Selective detection of human papilloma virus DNAs by specific synthetic DNA probes.

Cravador, A; Herzog, A; Houard, S; d'Ippolito, P; Carroll, R; Bollen, A

Cravador, A; Herzog, A; Houard, S; d'Ippolito, P; Carroll, R; Bollen, A.

Afiliación

Cravador A; Service of Applied Genetics, University of Brussels, Nivelles, Belgium.

Mol Cell Probes ; 3(2): 143-58, 1989 Jun.

Article en En | MEDLINE | ID: mdl-2549406

RESUMEN

Specific oligodeoxynucleotide probes ranging from 20 to 35 nucleotides were defined to differentiate each of the HPV1a, 5, 6b, 8, 11, 16, 18 and 33. They were chosen using computer programs developed to compare simultaneously several 8000 bp long DNA sequences. Sequences common to all and to specific groups of the HPV DNA were also selected. Specificity of 32P-labelled probes for HPV6b, 11, 16, 18 and 33 was demonstrated and the sensitivity of the assays was evaluated by filter hybridization with viral clones and with DNA from cervical tumor biopsies.

Asunto(s)

Sondas de ADN de HPV/análisis; Sondas de ADN/análisis; ADN Viral/análisis; Papillomaviridae/genética; Secuencia de Bases; Femenino; Humanos; Hibridación de Ácido Nucleico; Neoplasias del Cuello Uterino/análisis; Neoplasias del Cuello Uterino/microbiología

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papillomaviridae / ADN Viral / Sondas de ADN / Sondas de ADN de HPV Tipo de estudio: Diagnostic_studies Límite: Female / Humans Idioma: En Revista: Mol Cell Probes Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 1989 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido

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