Your browser doesn't support javascript.
loading
Radiation effects on DNA content of cervical cancer cells: A rapid evaluation of radiation sensitivity by laser scanning cytometry.
Fujiyoshi, Naoki; Ushijima, Kimio; Kawano, Kouichiro; Fujiyoshi, Keizo; Yamaguchi, Tomohiko; Araki, Yuko; Kakuma, Tatsuyuki; Watanabe, Sumiko; Kaku, Tsunehisa; Nishida, Takashi; Kamura, Toshiharu.
Afiliación
  • Fujiyoshi N; Department of Obstetrics and Gynecology, School of Medicine, Kurume University, Kurume, Fukuoka ; Department of Gynecology, Oita Prefecture Saiseikai Hita Hospital, Hita, Oita.
  • Ushijima K; Department of Obstetrics and Gynecology, School of Medicine, Kurume University, Kurume, Fukuoka.
  • Kawano K; Department of Obstetrics and Gynecology, School of Medicine, Kurume University, Kurume, Fukuoka.
  • Fujiyoshi K; Department of Obstetrics and Gynecology, School of Medicine, Kurume University, Kurume, Fukuoka.
  • Yamaguchi T; Department of Diagnostic Pathology, Kurume University Hospital, Kurume, Japan.
  • Araki Y; Department of Biostatistics, School of Medicine, Kurume University, Kurume, Japan.
  • Kakuma T; Department of Biostatistics, School of Medicine, Kurume University, Kurume, Japan.
  • Watanabe S; Department of Health Science, Faculty of Medicine, Kyushu University, Higashi-ku, Fukuoka, Japan.
  • Kaku T; Department of Health Science, Faculty of Medicine, Kyushu University, Higashi-ku, Fukuoka, Japan.
  • Nishida T; Department of Gynecology, Oita Prefecture Saiseikai Hita Hospital, Hita, Oita.
  • Kamura T; Department of Obstetrics and Gynecology, School of Medicine, Kurume University, Kurume, Fukuoka.
Mol Clin Oncol ; 3(1): 51-54, 2015 Jan.
Article en En | MEDLINE | ID: mdl-25469269
Since uterine cervical cancer is regarded as a radiosensive tumor, ionizing radiation is the most frequently used treatment modality against the disease. Although the crucial end-point is radiation-induced cell death, the tumors are not equally sensitive to radiation. Determining the criteria that may be used to predict tumor radiosensitivity is of importance; however, little success has been achieved thus far. In radioresistant cases the therapeutic strategy should be changed, thereby avoiding ineffective or unnecessary treatment. Furthermore, identification of the underlying molecular processes leading to radioresistance may lead to novel radiosensitising strategies. Cervical smears were obtained from seven patients with locally advanced cervical cancer following each radiotherapy, and the radiation-induced damage of cancer tissue was examined by routine cytology. Since the formation of DNA double-strand breaks is considered critical for the cytocidal effect of radiation therapy, the molecular changes of the neoplastic cells were also assessed by laser scanning cytometry (LSC). Radiation-induced morphological changes of cancer cells were evident at a dose of 7.2 Gy, whereas increased DNA content (or DNA index) was observed prior to the onset of morphological changes. Molecular change was detected earlier than the morphological change of the irradiated cancer cells, indicating the feasibility of LSC in predicting the radiosensitivity of cervical cancer tissue.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Mol Clin Oncol Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Mol Clin Oncol Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido