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Action of silver nanoparticles towards biological systems: cytotoxicity evaluation using hen's egg test and inhibition of Streptococcus mutans biofilm formation.
Freire, Priscila L L; Stamford, Thayza C M; Albuquerque, Allan J R; Sampaio, Fabio C; Cavalcante, Horacinna M M; Macedo, Rui O; Galembeck, André; Flores, Miguel A P; Rosenblatt, Aronita.
Afiliación
  • Freire PL; Faculty of Dentistry, University of Pernambuco, Av. Gal. Newton Cavalcanti, n° 1650, CEP: 54753-020, Camaragibe, PE, Brazil. Electronic address: perezila@hotmail.com.
  • Stamford TC; Federal University of Pernambuco, Av. Profª Morais Rego, n° 1235, Cidade Universitária, CEP: 50670-901, Recife, PE, Brazil.
  • Albuquerque AJ; Federal University of Paraíba, Campus Universitário I, Cidade Universitária, CEP: 58059-900, João Pessoa, PB, Brazil.
  • Sampaio FC; Federal University of Paraíba, Campus Universitário I, Cidade Universitária, CEP: 58059-900, João Pessoa, PB, Brazil.
  • Cavalcante HM; Federal University of Paraíba, Campus Universitário I, Cidade Universitária, CEP: 58059-900, João Pessoa, PB, Brazil.
  • Macedo RO; Federal University of Paraíba, Campus Universitário I, Cidade Universitária, CEP: 58059-900, João Pessoa, PB, Brazil.
  • Galembeck A; Federal University of Pernambuco, Av. Profª Morais Rego, n° 1235, Cidade Universitária, CEP: 50670-901, Recife, PE, Brazil; Center of Strategic Technologies of Northeast, Campus MCTI Nordeste, Av. Prof. Luiz Freire, n° 01, CEP: 50740-540, Recife, PE, Brazil.
  • Flores MA; Center of Strategic Technologies of Northeast, Campus MCTI Nordeste, Av. Prof. Luiz Freire, n° 01, CEP: 50740-540, Recife, PE, Brazil.
  • Rosenblatt A; Faculty of Dentistry, University of Pernambuco, Av. Gal. Newton Cavalcanti, n° 1650, CEP: 54753-020, Camaragibe, PE, Brazil.
Int J Antimicrob Agents ; 45(2): 183-7, 2015 Feb.
Article en En | MEDLINE | ID: mdl-25455849
This study aimed to evaluate the cytotoxicity and bactericidal properties of four silver nanoparticle (AgNP) colloids and their ability to inhibit Streptococcus mutans biofilm formation on dental enamel. The cytotoxicity of AgNPs was evaluated based on signs of vascular change on the chorioallantoic membrane using the hen's egg test (HET-CAM). Bactericidal properties and inhibition of S. mutans biofilm formation were determined using a parallel-flow cell system and a dichromatic fluorescent stain. The percentage of viable cells was calculated from regression data generated from a viability standard. AgNP colloids proved to be non-irritating, as they were unable to promote vasoconstriction, haemorrhage or coagulation. AgNP colloids inhibited S. mutans biofilm formation on dental enamel, and cell viability measured by fluorescence was 0% for samples S1, S2, S3 and S4 and 36.5% for the positive control (diluted 30% silver diamine fluoride). AgNPs are new products with a low production cost because they have a lower concentration of silver, with low toxicity and an effective bactericidal effect against a cariogenic oral bacterium. Moreover, they do not promote colour change in dental enamel, which is an aesthetic advantage compared with traditional silver products.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / Streptococcus mutans / Biopelículas / Nanopartículas del Metal Límite: Animals Idioma: En Revista: Int J Antimicrob Agents Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / Streptococcus mutans / Biopelículas / Nanopartículas del Metal Límite: Animals Idioma: En Revista: Int J Antimicrob Agents Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos