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Bovine herpesvirus-1: evaluation of genetic diversity of subtypes derived from field strains of varied clinical syndromes and their relationship to vaccine strains.
Fulton, R W; d'Offay, J M; Eberle, R; Moeller, R B; Campen, H Van; O'Toole, D; Chase, C; Miller, M M; Sprowls, R; Nydam, D V.
Afiliación
  • Fulton RW; Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078, USA. Electronic address: Robert.fulton@okstate.edu.
  • d'Offay JM; Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078, USA.
  • Eberle R; Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078, USA.
  • Moeller RB; California Animal Health and Food Safety Laboratory, Tulare, CA 93274, USA.
  • Campen HV; Colorado State University Veterinary Diagnostic Laboratory, Fort Collins, CO 80523, USA.
  • O'Toole D; Wyoming State Veterinary Laboratory, Laramie, WY 82070, USA.
  • Chase C; South Dakota State University, Brookings, SD 57007, USA.
  • Miller MM; Wyoming State Veterinary Laboratory, Laramie, WY 82070, USA.
  • Sprowls R; Texas A&M Veterinary Medical Diagnostic Laboratory, Amarillo, TX 79106, USA.
  • Nydam DV; Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USA.
Vaccine ; 33(4): 549-58, 2015 Jan 15.
Article en En | MEDLINE | ID: mdl-25454086
Bovine herpesvirus-1 (BoHV-1) causes significant disease in cattle. Control programs in North America incorporate vaccination with modified live viral (MLV) or killed (KV) vaccine. BoHV-1 strains are isolated from diseased animals or fetuses after vaccination. There are markers for differentiating MLV from field strains using whole-genome sequencing and analysis identifying single nucleotide polymorphisms (SNPs). Using multiple primer sets and sequencing of products permits association of BoHV-1 isolates with vaccines. To determine association between vaccine virus and strains isolated from clinical cases following vaccination, we analyzed 12 BoHV-1 isolates from animals with various clinical syndromes; 9 corresponded to BoHV-1.1 respiratory group. The remaining three corresponded to BoHV-1.2b, typically found in genital tracts of cattle. Four BoHV-1 isolates were identical to a vaccine strain; three were from post-vaccination abortion episodes with typical herpetic lesions whose dams had received MLV vaccine during pregnancy, and one from a heifer given a related MLV vaccine; Sequences of two respiratory isolates perfectly matched mutations characterizing RLB106 strain, a temperature sensitive mutant used in intranasal and parenteral vaccines. The last three respiratory strains clearly appeared related to a group of MLV vaccines. Previously the MLV vaccines were grouped into four groups based on SNPs patterns. In contrast with above-mentioned isolates that closely matched SNP patterns of their respective MLV vaccine virus, these 3 strains both lacked some and possessed a number of additional mutations compared to a group of MLV vaccine viral genome. Finding BoHV-1.2b in respiratory cases indicates focus should be given BoHV-1.2b as an emerging virus or a virus not recognized nor fully characterized in BRD.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Variación Genética / Enfermedades de los Bovinos / Infecciones por Herpesviridae / Herpesvirus Bovino 1 / Vacunas contra Herpesvirus Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy País/Región como asunto: America do norte Idioma: En Revista: Vaccine Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Variación Genética / Enfermedades de los Bovinos / Infecciones por Herpesviridae / Herpesvirus Bovino 1 / Vacunas contra Herpesvirus Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy País/Región como asunto: America do norte Idioma: En Revista: Vaccine Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos