Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.
Am J Reprod Immunol
; 73(4): 372-82, 2015 Apr.
Article
en En
| MEDLINE
| ID: mdl-25427847
PROBLEM: The chemokine IL-8 recruits neutrophils to sites of infection, including the endometrium of the bovine uterus. However, quantification of bovine IL-8 often yields lower concentrations than for other species, which may reflect impaired innate immune responses by bovine cells or inaccurate measurement of IL-8 using the current human IL-8 ELISA method. METHOD OF STUDY: An ELISA was developed and validated for detection of bovine IL-8. Utility of the assay was tested by measuring the response of bovine endometrium and cells to bacteria and pathogen-associated molecular patterns. RESULTS: The developed ELISA detected 62.5-2000 pg/mL IL-8, with minimal cross-reactivity to other inflammatory mediators. Concentrations of bovine IL-8 were measured more accurately by the bovine than human IL-8 ELISA. Bovine endometrial IL-8 responses to pathogen-associated molecules were quantitatively similar to other species. CONCLUSION: A bovine-specific IL-8 ELISA was developed, which accurately measured IL-8 secretion from endometrial cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Interleucina-8
/
Infecciones del Sistema Genital
/
Genitales Femeninos
Límite:
Animals
Idioma:
En
Revista:
Am J Reprod Immunol
Año:
2015
Tipo del documento:
Article
Pais de publicación:
Dinamarca