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Chimerism analysis in peripheral blood using indel quantitative real-time PCR is a useful tool to predict post-transplant relapse in acute leukemia.
Jacque, N; Nguyen, S; Golmard, J-L; Uzunov, M; Garnier, A; Leblond, V; Vernant, J-P; Bories, D; Dhédin, N.
Afiliación
  • Jacque N; Hematology Department, Pitié-Salpêtrière Hospital, Paris, France.
  • Nguyen S; Hematology Department, Pitié-Salpêtrière Hospital, Paris, France.
  • Golmard JL; Biostatistics Department, Pitié-Salpêtrière Hospital, Paris, France.
  • Uzunov M; Hematology Department, Pitié-Salpêtrière Hospital, Paris, France.
  • Garnier A; Hematology Department, Pitié-Salpêtrière Hospital, Paris, France.
  • Leblond V; Hematology Department, Pitié-Salpêtrière Hospital, Paris, France.
  • Vernant JP; Hematology Department, Pitié-Salpêtrière Hospital, Paris, France.
  • Bories D; Molecular Hematology Unit, Henri Mondor Hospital, Créteil, France.
  • Dhédin N; 1] Hematology Department, Pitié-Salpêtrière Hospital, Paris, France [2] Adolescent Young Adult Unit, Hematology Department, Saint Louis Hospital, Paris, France.
Bone Marrow Transplant ; 50(2): 259-65, 2015 Feb.
Article en En | MEDLINE | ID: mdl-25387089
Detection of increasing mixed chimerism (IMC) using standard PCR correlates with relapse after allo-SCT for acute leukemias (ALs). Quantitative real-time PCR of insertion/deletion polymorphism (indel qrtPCR) is a much more sensitive method, which can be performed on peripheral blood. We studied the significance of low increases of recipient cells (0.1%) detected by indel qrtPCR in a cohort of 89 transplants. We did not observe relapse among the 32 patients with no IMC. Fifty-seven patients presented a first IMC, which was followed by four different scenarios: a decreasing MC (26 cases, no relapse), a stable MC (1 case, 1 relapse), a second IMC (24 cases, 15 relapse) or no control of chimerism (6 cases, 5 relapses). In multivariate analysis, detection of two successive IMCs was strongly associated with relapse (hazard ratio (HR): 9.4, 95% confidence interval (CI): 3.8-23; P<0.0001). Among the 57 patients who presented at least one IMC, 27 underwent immunomodulation (tapering of immunosuppression or donor lymphocyte injection), leading to a 1-year relapse rate of 15.7% vs 57.6% in the 30 other patients (P=0.0007). Altogether, these results indicate that chimerism analysis using indel qrtPCR in peripheral blood is a useful tool for detection of relapse in patients transplanted for AL.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / Quimera por Trasplante / Trasplante de Células Madre / Leucemia-Linfoma Linfoblástico de Células Precursoras / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Bone Marrow Transplant Asunto de la revista: TRANSPLANTE Año: 2015 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / Quimera por Trasplante / Trasplante de Células Madre / Leucemia-Linfoma Linfoblástico de Células Precursoras / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Bone Marrow Transplant Asunto de la revista: TRANSPLANTE Año: 2015 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido