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Modulation of rotation-induced lift force for cell filtration in a low aspect ratio microchannel.
Zhou, Jian; Giridhar, Premkumar Vummidi; Kasper, Susan; Papautsky, Ian.
Afiliación
  • Zhou J; BioMicroSystems Lab, Department of Electrical Engineering and Computing Systems, University of Cincinnati , Cincinnati, Ohio 45221, USA.
  • Giridhar PV; Department of Environmental Health, College of Medicine, University of Cincinnati , Cincinnati, Ohio 45221, USA.
  • Kasper S; Department of Environmental Health, College of Medicine, University of Cincinnati , Cincinnati, Ohio 45221, USA.
  • Papautsky I; BioMicroSystems Lab, Department of Electrical Engineering and Computing Systems, University of Cincinnati , Cincinnati, Ohio 45221, USA.
Biomicrofluidics ; 8(4): 044112, 2014 Jul.
Article en En | MEDLINE | ID: mdl-25379097
Cell filtration is a critical step in sample preparation in many bioapplications. Herein, we report on a simple, filter-free, microfluidic platform based on hydrodynamic inertial migration. Our approach builds on the concept of two-stage inertial migration which permits precise prediction of microparticle position within the microchannel. Our design manipulates equilibrium positions of larger microparticles by modulating rotation-induced lift force in a low aspect ratio microchannel. Here, we demonstrate filtration of microparticles with extreme efficiency (>99%). Using multiple prostate cell lines (LNCaP and human prostate epithelial tumor cells), we show filtration from spiked blood, with 3-fold concentration and >83% viability. Results of a proliferation assay show normal cell division and suggest no negative effects on intrinsic properties. Considering the planar low-aspect-ratio structure and predictable focusing, we envision promising applications and easy integration with existing lab-on-a-chip systems.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomicrofluidics Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomicrofluidics Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos