Supercoil-stabilized left-handed DNA in the plasmid (dA-dT)16 insert formed in the presence of Ni2+.
FEBS Lett
; 243(2): 313-7, 1989 Jan 30.
Article
en En
| MEDLINE
| ID: mdl-2537234
The (dA-dT)16 insert of the plasmid pAT32 was probed with diethyl pyrocarbonate (DEPC) and nuclease Bal3l in the presence of Ni2+ known to be able to induce transition to left-handed conformation in the synthetic poly(dA-dT).poly(dA-T). It has been shown that this insert in a supercoiled plasmid displays a DEPC modification pattern characteristic of left-handed DNA under conditions not sufficient to induce a left-handed structure in the linear plasmid and poly(dA-dT).poly(dA-T).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Poli dA-dT
/
Polidesoxirribonucleótidos
/
ADN Superhelicoidal
/
Elementos Transponibles de ADN
/
Níquel
Idioma:
En
Revista:
FEBS Lett
Año:
1989
Tipo del documento:
Article
Pais de publicación:
Reino Unido