Seamless stitching of biosynthetic gene cluster containing type I polyketide synthases using Red/ET mediated recombination for construction of stably co-existing plasmids.

Su, Chun; Zhao, Xin-Qing; Wang, Hai-Na; Qiu, Rong-Guo; Tang, Li

Su, Chun; Zhao, Xin-Qing; Wang, Hai-Na; Qiu, Rong-Guo; Tang, Li.

Afiliación

Su C; Research Center for Molecular Medicine, Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China.
Zhao XQ; Research Center for Molecular Medicine, Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China; School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
Wang HN; School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
Qiu RG; Research Center for Molecular Medicine, Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China; Beijing Biostar Technologies, Ltd., Beijing 101111, China.
Tang L; Research Center for Molecular Medicine, Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China; Beijing Biostar Technologies, Ltd., Beijing 101111, China. Electronic address: tangli63b@yahoo.com.

Gene ; 554(2): 233-40, 2015 Jan 10.

Article en En | MEDLINE | ID: mdl-25311549

Asunto(s)

Clonación Molecular/métodos; Escherichia coli/genética; Escherichia coli/metabolismo; Plásmidos/genética; Sintasas Poliquetidas/genética; Escherichia coli/enzimología; Proteínas de Escherichia coli/genética; Vectores Genéticos; Familia de Multigenes; Sintasas Poliquetidas/metabolismo; Recombinación Genética; Proteína Ribosómica S9; Proteínas Ribosómicas/genética; Análisis de Secuencia de ADN

Palabras clave

Co-existing; Heterologous expression; Polyketide synthases (PKS); Precise seamless stitching; Red/ET recombination

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásmidos / Clonación Molecular / Sintasas Poliquetidas / Escherichia coli Idioma: En Revista: Gene Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

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