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Comparison of innate immune agonists for induction of tracheal antimicrobial peptide gene expression in tracheal epithelial cells of cattle.
Berghuis, Lesley; Abdelaziz, Khaled Taha; Bierworth, Jodi; Wyer, Leanna; Jacob, Gabriella; Karrow, Niel A; Sharif, Shayan; Clark, Mary Ellen; Caswell, Jeff L.
Afiliación
  • Berghuis L; Department of Pathobiology, University of Guelph, Guelph, ON, N1G 2 W1, Canada. lesleymb@mun.ca.
  • Abdelaziz KT; Department of Pathobiology, University of Guelph, Guelph, ON, N1G 2 W1, Canada. abdelazk@uoguelph.ca.
  • Bierworth J; Pathology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt. abdelazk@uoguelph.ca.
  • Wyer L; Department of Pathobiology, University of Guelph, Guelph, ON, N1G 2 W1, Canada. jbierwor@uoguelph.ca.
  • Jacob G; Department of Pathobiology, University of Guelph, Guelph, ON, N1G 2 W1, Canada. lwyer@uoguelph.ca.
  • Karrow NA; Department of Pathobiology, University of Guelph, Guelph, ON, N1G 2 W1, Canada. jacobg@uoguelph.ca.
  • Sharif S; Department of Animal and Poultry Science, University of Guelph, Guelph, ON, N1G 2 W1, Canada. nkarrow@uoguelph.ca.
  • Clark ME; Department of Pathobiology, University of Guelph, Guelph, ON, N1G 2 W1, Canada. shayan@uoguelph.ca.
  • Caswell JL; Department of Pathobiology, University of Guelph, Guelph, ON, N1G 2 W1, Canada. meclark@uoguelph.ca.
Vet Res ; 45: 105, 2014 Oct 12.
Article en En | MEDLINE | ID: mdl-25304258
Bovine respiratory disease is a complex of bacterial and viral infections of economic and welfare importance to the beef industry. Although tracheal antimicrobial peptide (TAP) has microbicidal activity against bacterial pathogens causing bovine respiratory disease, risk factors for bovine respiratory disease including BVDV and stress (glucocorticoids) have been shown to inhibit the induced expression of this gene. Lipopolysaccharide is known to stimulate TAP gene expression, but the maximum effect is only observed after 16 h of stimulation. The present study investigated other agonists of TAP gene expression in primary cultures of bovine tracheal epithelial cells. PCR analysis of unstimulated tracheal epithelial cells, tracheal tissue and lung tissue each showed mRNA expression for Toll-like receptors (TLRs) 1-10. Quantitative RT-PCR analysis showed that Pam3CSK4 (an agonist of TLR1/2) and interleukin (IL)-17A significantly induced TAP gene expression in tracheal epithelial cells after only 4-8 h of stimulation. Flagellin (a TLR5 agonist), lipopolysaccharide and interferon-α also had stimulatory effects, but little or no response was found with class B CpG ODN 2007 (TLR9 agonist) or lipoteichoic acid (TLR2 agonist). The use of combined agonists had little or no enhancing effect above that of single agonists. Thus, Pam3CSK4, IL-17A and lipopolysaccharide rapidly and significantly induce TAP gene expression, suggesting that these stimulatory pathways may be of value for enhancing innate immunity in feedlot cattle at times of susceptibility to disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Bovinos / Regulación de la Expresión Génica / Péptidos Catiónicos Antimicrobianos / Células Epiteliales / Receptores Toll-Like Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2014 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Bovinos / Regulación de la Expresión Génica / Péptidos Catiónicos Antimicrobianos / Células Epiteliales / Receptores Toll-Like Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2014 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido