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Optical suppression of drug-evoked phasic dopamine release.
McCutcheon, James E; Cone, Jackson J; Sinon, Christopher G; Fortin, Samantha M; Kantak, Pranish A; Witten, Ilana B; Deisseroth, Karl; Stuber, Garret D; Roitman, Mitchell F.
Afiliación
  • McCutcheon JE; Department of Psychology, University of Illinois at Chicago Chicago, IL, USA.
  • Cone JJ; Department of Psychology, University of Illinois at Chicago Chicago, IL, USA.
  • Sinon CG; Department of Psychology, University of Illinois at Chicago Chicago, IL, USA.
  • Fortin SM; Department of Psychology, University of Illinois at Chicago Chicago, IL, USA.
  • Kantak PA; Department of Psychiatry and Department of Cell Biology and Physiology, University of North Carolina Chapel Hill, NC, USA.
  • Witten IB; Princeton Neuroscience Institute and Department of Psychology, Princeton University Princeton, NJ, USA.
  • Deisseroth K; Departments of Bioengineering, Psychiatry and Behavioral Sciences, Howard Hughes Medical Institute, and CNC Program, Stanford University Stanford, CA, USA.
  • Stuber GD; Department of Psychiatry and Department of Cell Biology and Physiology, University of North Carolina Chapel Hill, NC, USA.
  • Roitman MF; Department of Psychology, University of Illinois at Chicago Chicago, IL, USA.
Front Neural Circuits ; 8: 114, 2014.
Article en En | MEDLINE | ID: mdl-25278845
Brief fluctuations in dopamine concentration (dopamine transients) play a key role in behavior towards rewards, including drugs of abuse. Drug-evoked dopamine transients may result from actions at both dopamine cell bodies and dopamine terminals. Inhibitory opsins can be targeted to dopamine neurons permitting their firing activity to be suppressed. However, as dopamine transients can become uncoupled from firing, it is unknown whether optogenetic hyperpolarization at the level of the soma is able to suppress dopamine transients. Here, we used in vivo fast-scan cyclic voltammetry to record transients evoked by cocaine and raclopride in nucleus accumbens (NAc) of urethane-anesthetized rats. We targeted halorhodopsin (NpHR) specifically to dopamine cells by injecting Cre-inducible virus into ventral tegmental area (VTA) of transgenic rats that expressed Cre recombinase under control of the tyrosine hydroxylase promoter (TH-Cre(+) rats). Consistent with previous work, co-administration of cocaine and raclopride led to the generation of dopamine transients in NAc shell. Illumination of VTA with laser strongly suppressed the frequency of transients in NpHR-expressing rats, but not in control rats. Laser did not have any effect on amplitude of transients. Thus, optogenetics can effectively reduce the occurrence of drug-evoked transients and is therefore a suitable approach for studying the functional role of such transients in drug-associated behavior.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dopamina / Área Tegmental Ventral / Optogenética / Núcleo Accumbens Límite: Animals Idioma: En Revista: Front Neural Circuits Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dopamina / Área Tegmental Ventral / Optogenética / Núcleo Accumbens Límite: Animals Idioma: En Revista: Front Neural Circuits Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza