Your browser doesn't support javascript.
loading
Loss of BMAL1 in ovarian steroidogenic cells results in implantation failure in female mice.
Liu, Yan; Johnson, Brian P; Shen, Anna L; Wallisser, Jacqueline A; Krentz, Kathy J; Moran, Susan M; Sullivan, Ruth; Glover, Edward; Parlow, Albert F; Drinkwater, Norman R; Schuler, Linda A; Bradfield, Christopher A.
Afiliación
  • Liu Y; McArdle Laboratory for Cancer Research, and Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706;
  • Johnson BP; McArdle Laboratory for Cancer Research, and Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706;
  • Shen AL; McArdle Laboratory for Cancer Research, and Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706;
  • Wallisser JA; McArdle Laboratory for Cancer Research, and Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706;
  • Krentz KJ; McArdle Laboratory for Cancer Research, and Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706;
  • Moran SM; McArdle Laboratory for Cancer Research, and Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706;
  • Sullivan R; McArdle Laboratory for Cancer Research, and Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706; Research Animal Resources Center, University of Wisconsin-Madison, Madison, WI 53706; and.
  • Glover E; McArdle Laboratory for Cancer Research, and Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706;
  • Parlow AF; National Hormone and Peptide Program, Harbor-University of California Los Angeles Medical Center, Torrance, CA 90502.
  • Drinkwater NR; McArdle Laboratory for Cancer Research, and Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706;
  • Schuler LA; Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706; Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison WI 53706;
  • Bradfield CA; McArdle Laboratory for Cancer Research, and Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison WI 53706; bradfield@oncology.wisc.edu.
Proc Natl Acad Sci U S A ; 111(39): 14295-300, 2014 Sep 30.
Article en En | MEDLINE | ID: mdl-25225411
The circadian clock plays a significant role in many aspects of female reproductive biology, including estrous cycling, ovulation, embryonic implantation, onset of puberty, and parturition. In an effort to link cell-specific circadian clocks to their specific roles in female reproduction, we used the promoter that controls expression of Steroidogenic Factor-1 (SF1) to drive Cre-recombinase-mediated deletion of the brain muscle arnt-like 1 (Bmal1) gene, known to encode an essential component of the circadian clock (SF1-Bmal1(-/-)). The resultant SF1-Bmal1(-/-) females display embryonic implantation failure, which is rescued by progesterone supplementation, or bilateral or unilateral transplantation of wild-type ovaries into SF1-Bmal1(-/-) dams. The observation that the central clock, and many other peripheral clocks, are fully functional in this model allows the assignment of the implantation phenotype to the clock in ovarian steroidogenic cells and distinguishes it from more general circadian related systemic pathology (e.g., early onset arthropathy, premature aging, ovulation, late onset of puberty, and abnormal estrous cycle). Our ovarian transcriptome analysis reveals that deletion of ovarian Bmal1 disrupts expression of transcripts associated with the circadian machinery and also genes critical for regulation of progesterone production, such as steroidogenic acute regulatory factor (Star). Overall, these data provide a powerful model to probe the interlocking and synergistic network of the circadian clock and reproductive systems.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Implantación del Embrión / Esteroides / Factores de Transcripción ARNTL Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Implantación del Embrión / Esteroides / Factores de Transcripción ARNTL Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos