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Chitosan-alginate membranes accelerate wound healing.
Caetano, Guilherme Ferreira; Frade, Marco Andrey Cipriani; Andrade, Thiago Antônio Moretti; Leite, Marcel Nani; Bueno, Cecilia Zorzi; Moraes, Ângela Maria; Ribeiro-Paes, João Tadeu.
Afiliación
  • Caetano GF; Biotecnology Post Graduate Program, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, São Paulo, Brazil.
  • Frade MA; Department of Internal Medicine, Division of Dermatology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
  • Andrade TA; Department of Internal Medicine, Division of Dermatology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
  • Leite MN; Department of Internal Medicine, Division of Dermatology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
  • Bueno CZ; Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, State University of Campinas, Campinas, São Paulo, Brazil.
  • Moraes ÂM; Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, State University of Campinas, Campinas, São Paulo, Brazil.
  • Ribeiro-Paes JT; Department of Biology, São Paulo State University (UNESP), Assis, São Paulo, Brazil.
J Biomed Mater Res B Appl Biomater ; 103(5): 1013-22, 2015 Jul.
Article en En | MEDLINE | ID: mdl-25220821
The purpose of this study was to evaluate the efficacy of chitosan-alginate membrane to accelerate wound healing in experimental cutaneous wounds. Two wounds were performed in Wistar rats by punching (1.5 cm diameter), treated with membranes moistened with saline solution (CAM group) or with saline only (SL group). After 2, 7, 14, and 21 days of surgery, five rats of each group were euthanized and reepithelialization was evaluated. The wounds/scars were harvested for histological, flow cytometry, neutrophil infiltrate, and hydroxyproline analysis. CAM group presented higher inflammatory cells recruitment as compared to SL group on 2(nd) day. On the 7(th) day, CAM group showed higher CD11b(+) level and lower of neutrophils than SL group. The CAM group presented higher CD4(+) cells influx than SL group on 2(nd) day, but it decreased during the follow up and became lower on 14(th) and 21(st) days. Higher fibroplasia was noticed on days 7 and 14 as well as higher collagenesis on 21(st) in the CAM group in comparison to SL group. CAM group showed faster reepithelialization on 7(th) day than SL group, although similar in other days. In conclusion, chitosan-alginate membrane modulated the inflammatory phase, stimulated fibroplasia and collagenesis, accelerating wound healing process in rats.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Quitosano / Alginatos / Membranas Artificiales Límite: Animals Idioma: En Revista: J Biomed Mater Res B Appl Biomater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2015 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Quitosano / Alginatos / Membranas Artificiales Límite: Animals Idioma: En Revista: J Biomed Mater Res B Appl Biomater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2015 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos