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Class I HDAC inhibition stimulates cardiac protein SUMOylation through a post-translational mechanism.
Blakeslee, Weston W; Wysoczynski, Christina L; Fritz, Kristofer S; Nyborg, Jennifer K; Churchill, Mair E A; McKinsey, Timothy A.
Afiliación
  • Blakeslee WW; Department of Medicine, Division of Cardiology, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA; Department of Pharmacology, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.
  • Wysoczynski CL; Department of Pharmacology, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.
  • Fritz KS; Department of Pharmaceutical Sciences, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.
  • Nyborg JK; Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, USA.
  • Churchill ME; Department of Pharmacology, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.
  • McKinsey TA; Department of Medicine, Division of Cardiology, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA; Department of Pharmacology, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA. Electronic address: timothy.mckinsey@ucdenver.edu.
Cell Signal ; 26(12): 2912-20, 2014 Dec.
Article en En | MEDLINE | ID: mdl-25220405
Lysine residues are subject to a multitude of reversible post-translational modifications, including acetylation and SUMOylation. In the heart, enhancement of lysine acetylation or SUMOylation using histone deacetylase (HDAC) inhibitors or SUMO-1 gene transfer, respectively, has been shown to be cardioprotective. Here, we addressed whether there is crosstalk between lysine acetylation and SUMOylation in the heart. Treatment of cardiomyocytes and cardiac fibroblasts with pharmacological inhibitors of HDAC catalytic activity robustly increased conjugation of SUMO-1, but not SUMO-2/3, to several high molecular weight proteins in both cell types. The use of a battery of selective HDAC inhibitors and short hairpin RNAs demonstrated that HDAC2, which is a class I HDAC, is the primary HDAC isoform that controls cardiac protein SUMOylation. HDAC inhibitors stimulated protein SUMOylation in the absence of de novo gene transcription or protein synthesis, revealing a post-translational mechanism of HDAC inhibitor action. HDAC inhibition did not suppress the activity of de-SUMOylating enzymes, suggesting that increased protein SUMOylation in HDAC inhibitor-treated cells is due to stimulation of SUMO-1 conjugation rather than blockade of SUMO-1 cleavage. Consistent with this, multiple components of the SUMO conjugation machinery were capable of being acetylated in vitro. These findings reveal a novel role for reversible lysine acetylation in the control of SUMOylation in the heart, and suggest that cardioprotective actions of HDAC inhibitors are in part due to stimulation of protein SUMO-1-ylation in myocytes and fibroblasts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteína SUMO-1 / Miocitos Cardíacos / Inhibidores de Histona Desacetilasas / Sumoilación Límite: Animals / Humans Idioma: En Revista: Cell Signal Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteína SUMO-1 / Miocitos Cardíacos / Inhibidores de Histona Desacetilasas / Sumoilación Límite: Animals / Humans Idioma: En Revista: Cell Signal Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido