Thymosin ß4 induces invasion and migration of human colorectal cancer cells through the ILK/AKT/ß-catenin signaling pathway.

Piao, Zhengri; Hong, Chang-Soo; Jung, Mi-Ran; Choi, Chan; Park, Young-Kyu

Piao, Zhengri; Hong, Chang-Soo; Jung, Mi-Ran; Choi, Chan; Park, Young-Kyu.

Afiliación

Piao Z; Research Center for Molecular Therapeutic to GI Tract of Cancer Center, Chonnam National University Hwasun Hospital, Hwasun, Republic of Korea; Center for Creative Biomedical Scientists (BK-21 Plus Project), Chonnam National University Medical School, Gwangju, Republic of Korea.
Hong CS; Research Center for Molecular Therapeutic to GI Tract of Cancer Center, Chonnam National University Hwasun Hospital, Hwasun, Republic of Korea.
Jung MR; Department of Gastroenterologic Surgery, Chonnam National University Hwasun Hospital, Hwasun, Republic of Korea.
Choi C; Department of Pathology, Chonnam National University Hwasun Hospital, Hwasun, Republic of Korea.
Park YK; Research Center for Molecular Therapeutic to GI Tract of Cancer Center, Chonnam National University Hwasun Hospital, Hwasun, Republic of Korea; Center for Creative Biomedical Scientists (BK-21 Plus Project), Chonnam National University Medical School, Gwangju, Republic of Korea; Department of Gastro

Biochem Biophys Res Commun ; 452(3): 858-64, 2014 Sep 26.

Article en En | MEDLINE | ID: mdl-25218472

RESUMEN

Thymosin ß4 (Tß4) is a 43-amino-acid peptide involved in many biological processes. However, the precise molecular signaling mechanism(s) of Tß4 in cell invasion and migration remain unclear. In this study, we show that Tß4 was significantly overexpressed in colorectal cancer tissues compared to adjacent normal tissues and high levels of Tß4 were correlated with stage of colorectal cancer, and that Tß4 expression was associated with morphogenesis and EMT. Tß4-upregulated cancer cells showed increased adhesion, invasion and migration activity, whereas Tß4-downregulated cells showed decreased activities. We also demonstrated that Tß4 interacts with ILK, which promoted the phosphorylation and activation of AKT, the phosphorylation and inactivation of GSK3ß, the expression and nuclear localization of ß-catenin, and integrin receptor activation. These results suggest that Tß4 is an important regulator of the ILK/AKT/ß-catenin/Integrin signaling cascade to induce cell invasion and migration in colorectal cancer cells, and is a potential target for cancer treatment.

Asunto(s)

Neoplasias Colorrectales/genética; Regulación Neoplásica de la Expresión Génica; Proteínas Serina-Treonina Quinasas/genética; Proteínas Proto-Oncogénicas c-akt/genética; Timosina/genética; beta Catenina/genética; Anciano; Línea Celular Tumoral; Movimiento Celular; Neoplasias Colorrectales/metabolismo; Neoplasias Colorrectales/patología; Transición Epitelial-Mesenquimal; Femenino; Glucógeno Sintasa Quinasa 3/genética; Glucógeno Sintasa Quinasa 3/metabolismo; Glucógeno Sintasa Quinasa 3 beta; Humanos; Integrinas/genética; Integrinas/metabolismo; Metástasis Linfática; Masculino; Persona de Mediana Edad; Invasividad Neoplásica; Estadificación de Neoplasias; Proteínas Serina-Treonina Quinasas/metabolismo; Proteínas Proto-Oncogénicas c-akt/metabolismo; Transducción de Señal; Timosina/metabolismo; Microambiente Tumoral; beta Catenina/metabolismo

Palabras clave

Adhesion; Colorectal cancer; Invasion; Migration; Thymosin ß4

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Timosina / Neoplasias Colorrectales / Regulación Neoplásica de la Expresión Génica / Proteínas Serina-Treonina Quinasas / Proteínas Proto-Oncogénicas c-akt / Beta Catenina Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Biochem Biophys Res Commun Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos

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