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The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis.
dos Santos Aliança, Amanda Silva; dos Anjos, Keicyanne Fernanda Lessa; de Vasconcelos Reis, Thiago Nogueira; Higino, Taciana Mirely Maciel; Brelaz-de-Castro, Maria Carolina Accioly; Bianco, Éverson Miguel; de Figueiredo, Regina Celia Bressan Queiroz.
Afiliación
  • dos Santos Aliança AS; Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhães (CPqAM-FIOCRUZ), Av. Moraes Rego s/n Cidade Universitária, Campus da UFPE, Recife 50670-420, Brazil. Amanda_alianca@yahoo.com.br.
  • dos Anjos KF; Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhães (CPqAM-FIOCRUZ), Av. Moraes Rego s/n Cidade Universitária, Campus da UFPE, Recife 50670-420, Brazil. keicyanne1@yahoo.com.br.
  • de Vasconcelos Reis TN; Departamento de Oceanografia, Universidade Federal de Pernambuco (UFPE), Recife 50740-550, Brazil. reistnv@gmail.com.
  • Higino TM; Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhães (CPqAM-FIOCRUZ), Av. Moraes Rego s/n Cidade Universitária, Campus da UFPE, Recife 50670-420, Brazil. taciana@cpqam.fiocruz.br.
  • Brelaz-de-Castro MC; Departamento de Imunologia Centro de Pesquisa Aggeu Magalhães (CPqAM-FIOCRUZ), Av. Moraes Rego s/n Cidade Universitária, Campus da UFPE, Recife 50670-420, Brazil. carolina.brelaz@gmail.com.
  • Bianco ÉM; Programa de Pós-graduação em Química, Fundação Universidade Regional de Blumenau (FURB), Campus 1, Rua Antonio da Veiga, 140, Blumenal 89012-900, Brazil. ebianco@chemist.com.
  • de Figueiredo RC; Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhães (CPqAM-FIOCRUZ), Av. Moraes Rego s/n Cidade Universitária, Campus da UFPE, Recife 50670-420, Brazil. bressan@cpqam.fiocruz.br.
Molecules ; 19(9): 14052-65, 2014 Sep 09.
Article en En | MEDLINE | ID: mdl-25207712
Seaweeds present a wide variety of interesting bioactive molecules. In the present work we evaluated the biological activity of the dichloromethane/methanol (2:1) extract (DME) from the brown seaweed Dictyota mertensii against Leishmania amazonensis and its cytotoxic potential on mammalian cells. The extract showed significant inhibitory effect on the growth of promastigote forms (IC50=71.60 µg/mL) and low toxicity against mammalian cells (CC50=233.10 µg/mL). The DME was also efficient in inhibiting the infection in macrophages, with CC50 of 81.4 µg/mL and significantly decreased the survival of amastigote forms within these cells. The selectivity index showed that DME was more toxic to both promastigote (SI=3.25) and amastigote (SI=2.86) forms than to macrophages. Increased NO production was observed in treated macrophages suggesting that besides acting directly on the parasites, the DME also shows an immunomodulatory effect on macrophages. Drastic ultrastructural alterations consistent with loss of viability and cell death were observed in treated parasites. Confocal microscopy and cytometry analyzes showed no significant impairment of plasma membrane integrity, whereas an intense depolarization of mitochondrial membrane could be observed by using propidium iodide and rhodamine 123 staining, respectively. The low toxicity to mammalian cells and the effective activity against promastigotes and amastigotes, point to the use of DME as a promising agent for the treatment of cutaneous leishmaniasis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leishmaniasis / Phaeophyceae / Leishmania / Antiprotozoarios Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leishmaniasis / Phaeophyceae / Leishmania / Antiprotozoarios Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza