PtLGBP, a pattern recognition receptor in Portunus trituberculatus involved in the immune response against different challenges.

Chen, Y-E; Jin, S; Zhao, Q-S; Zhang, Y; Wang, C-L

Chen, Y-E; Jin, S; Zhao, Q-S; Zhang, Y; Wang, C-L.

Afiliación

Chen YE; Ningbo University School of Marine Sciences Ningbo, Zhejiang 315211 China.
Jin S; Ningbo University School of Marine Sciences Ningbo, Zhejiang 315211 China.
Zhao QS; Ningbo University School of Marine Sciences Ningbo, Zhejiang 315211 China.
Zhang Y; Ningbo University School of Marine Sciences Ningbo, Zhejiang 315211 China.
Wang CL; Ningbo University School of Marine Sciences Ningbo, Zhejiang 315211 China.

Acta Biol Hung ; 65(3): 294-304, 2014 Sep.

Article en En | MEDLINE | ID: mdl-25194733

RESUMEN

Lipopolysaccharide and b-1,3-glucan binding protein (LGBP) is a pattern recognition receptor that can recognize and bind LPS and b-1,3-glucan. LGBP has crucial roles in innate immune defense against Gram-negative bacteria and fungi. In this study, LGBP functions in Portunus trituberculatus innate immunity were analyzed. First, the mRNA expression of PtLGBP in hemocytes, hepatopancreas, and muscle toward three typical pathogen-associated molecular patterns (PAMPs) stimulations were examined using real-time PCR. Results show that the overall trend of relative expressions of the LGBP gene in three tissues is consistent, showing up-down trend. In each group, the highest expression of the LGBP gene was at 3 and 12 h post-injection. The LGBP gene is also expressed significantly higher in the hemocytes and hepatopancreas than in the muscle. The highest level of LGBP was in the lipopolysaccharides (LPS) and glucan-injected group, whereas the lowest level was in the PGN-injected group. Furthermore, bacterial agglutination assay with polyclonal antibody specifically for PtLGBP proved that the recombinant PtLGBP (designated as rPtLGBP) could exhibit obvious agglutination activity toward Gram-negative bacteria Escherichia coli, Vibrio parahaemolyticus, and V. alginolyticus; Gram-positive bacteria Bacillus subtilis; and fungi Saccharomyces cerevisiae. LGBP in Portunus trituberculatus possibly served as a multi-functional PRR. In addition, LGBP is not only involved in the immune response against Gram-negative and fungi, as manifested in other invertebrates, but also has a significant role in anti-Gram-positive bacteria infection.

Asunto(s)

Braquiuros/efectos de los fármacos; Endotoxinas/farmacología; Inmunidad Innata/efectos de los fármacos; Lectinas/efectos de los fármacos; Peptidoglicano/farmacología; Receptores de Reconocimiento de Patrones/efectos de los fármacos; beta-Glucanos/farmacología; Aglutinación/efectos de los fármacos; Pruebas de Aglutinación; Animales; Western Blotting; Braquiuros/genética; Braquiuros/inmunología; Braquiuros/metabolismo; Escherichia coli; Regulación de la Expresión Génica; Hemocitos/efectos de los fármacos; Hemocitos/inmunología; Hemocitos/metabolismo; Hepatopáncreas/efectos de los fármacos; Hepatopáncreas/inmunología; Hepatopáncreas/metabolismo; Lectinas/genética; Lectinas/metabolismo; Músculos/efectos de los fármacos; Músculos/inmunología; Músculos/metabolismo; ARN Mensajero/metabolismo; Receptores de Reconocimiento de Patrones/genética; Receptores de Reconocimiento de Patrones/metabolismo; Saccharomyces cerevisiae; Staphylococcus aureus; Factores de Tiempo

Palabras clave

LGBP; Portunus trituberculatus; innate immunity; pattern recognition receptor

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peptidoglicano / Braquiuros / Beta-Glucanos / Endotoxinas / Receptores de Reconocimiento de Patrones / Inmunidad Innata / Lectinas Límite: Animals Idioma: En Revista: Acta Biol Hung Año: 2014 Tipo del documento: Article Pais de publicación: Hungria

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