Possibility of CTX-M-14 Gene Transfer from Shigella sonnei to a Commensal Escherichia coli Strain of the Gastroenteritis Microbiome.
Osong Public Health Res Perspect
; 5(3): 156-60, 2014 Jun.
Article
en En
| MEDLINE
| ID: mdl-25180148
OBJECTIVES: To investigated whether the CTX-M-14 gene could be transferred from a clinical Shigella sonnei strain to commensal Escherichia coli strain in the gastroenteritis microbiome. METHODS: E. coli strains were isolated from 30 stool samples of S. sonnei infected students in a gastroenteritis outbreak in 2004 and were characterized by antibiotic resistance analysis, in vitro conjugation and in vivo transfer of CTX-M-14 gene and molecular assays. RESULTS: One strain of Escherichia coli that had high levels of resistance to cefotaxime was isolated from a patient infected with S. sonnei. Isoelectric focusing showed that the E. coli and S. sonnei strains produced a ß-lactamase with an isoelectric point of 8.1. Moreover, polymerase chain reaction analysis indicated that both strains possessed the same DNA sequences for CTX-M-14. The results of in vitro and in vivo conjugation showed that the efficiency of CTX-M-14 transfer from S. sonnei to E. coli was similar to CTX-M-14 transfer between E. coli strains. CONCLUSION: The data suggest that the acquisition of the extended-spectrum ß-lactamases gene by pathogenic bacteria in the human intestinal tract to commensal microbiome bacteria can cause serious infectious diseases.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Osong Public Health Res Perspect
Año:
2014
Tipo del documento:
Article
Pais de publicación:
Corea del Sur