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Synergistic anabolic actions of hyaluronic acid and platelet-rich plasma on cartilage regeneration in osteoarthritis therapy.
Chen, Wei-Hong; Lo, Wen-Cheng; Hsu, Wei-Che; Wei, Hong-Jian; Liu, Hen-Yu; Lee, Chian-Her; Tina Chen, Szu-Yu; Shieh, Ying-Hua; Williams, David F; Deng, Win-Ping.
Afiliación
  • Chen WH; Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan; Graduate Institute of Biomedical Materials and Engineering, College of Oral, Medicine, Taipei Medical University, Taipei, Taiwan.
  • Lo WC; Department of Neurosurgery, Taipei Medical University Hospital, Taipei, Taiwan; School of medicine, Taipei Medical University, Taipei, Taiwan.
  • Hsu WC; Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan; Graduate Institute of Biomedical Materials and Engineering, College of Oral, Medicine, Taipei Medical University, Taipei, Taiwan.
  • Wei HJ; Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan; Graduate Institute of Biomedical Materials and Engineering, College of Oral, Medicine, Taipei Medical University, Taipei, Taiwan.
  • Liu HY; Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan; Graduate Institute of Biomedical Materials and Engineering, College of Oral, Medicine, Taipei Medical University, Taipei, Taiwan.
  • Lee CH; Department of Orthopaedics and Traumatology, Orthopaedic Research Center, Taipei Medical University Hospital, Taipei, Taiwan.
  • Tina Chen SY; Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan; Graduate Institute of Biomedical Materials and Engineering, College of Oral, Medicine, Taipei Medical University, Taipei, Taiwan.
  • Shieh YH; Department of Family Medicine, Taipei Medical University, Wan Fang Hospital, Taipei, Taiwan.
  • Williams DF; Wake Forest Institute of Regenerative Medicine, Winston-Salem, NC, USA.
  • Deng WP; Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan; Graduate Institute of Biomedical Materials and Engineering, College of Oral, Medicine, Taipei Medical University, Taipei, Taiwan. Electronic address: wpdeng@tmu.edu.tw.
Biomaterials ; 35(36): 9599-607, 2014 Dec.
Article en En | MEDLINE | ID: mdl-25176059
Osteoarthritis (OA) is a common disease associated with tissue inflammation, physical disability and imbalanced homeostasis in cartilage. For advanced treatments, biological approaches are currently focused on tissue regeneration and anti-inflammation. This study was undertaken to evaluate the therapeutic efficacies of hyaluronic acid (HA) and platelet-rich plasma (PRP) (HA+PRP) on OA. Articular chondrocytes were obtained from five OA patients. The optimal HA and PRP concentrations were evaluated by MTT assay. The expressions of chondrogenic and inflammatory genes were analyzed by RT-PCR. Signaling pathway was examined by immunoblotting and the expressions of OA pathology-related chemokines and cytokines was demonstrated by real-time PCR-based SuperArray. The therapeutic efficacies of HA+PRP were then demonstrated in 3D arthritic neo-cartilage and ACLT-OA model. Here we showed that HA+PRP could greatly retrieve pro-inflammatory cytokines-reduced articular chondrocytes proliferation and chondrogenic phenotypes, the mechanism of which involve the sequential activation of specific receptors CD44 and TGF-ßRII, downstream mediators Smad2/3 and Erk1/2, and the chondrogenic transcription factor SOX9. The real-time PCR-based SuperArray results also indicated that OA pathology-related chemokines and cytokines could be efficiently suppressed by HA+PRP. Moreover, the cartilaginous ECM could be retrieved from inflammation-induced degradation by HA+PRP in both 2D monolayer and 3D neo-cartilage model. Finally, the intra-articular injection of HA+PRP could strongly rescue the meniscus tear and cartilage breakdown and then decrease OA-related immune cells. The combination of HA+PRP can synergistically promote cartilage regeneration and inhibit OA inflammation. This study might offer an advanced and alternative OA treatment based on detailed regenerative mechanisms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoartritis / Adyuvantes Inmunológicos / Condrocitos / Plasma Rico en Plaquetas / Ácido Hialurónico / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Biomaterials Año: 2014 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoartritis / Adyuvantes Inmunológicos / Condrocitos / Plasma Rico en Plaquetas / Ácido Hialurónico / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Biomaterials Año: 2014 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Países Bajos