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Monitoring the progression of loop-mediated isothermal amplification using conductivity.
Zhang, Xuzhi; Liu, Wenwen; Lu, Xun; Justin Gooding, J; Li, Qiufen; Qu, Keming.
Afiliación
  • Zhang X; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, People's Republic of China. Electronic address: zhangxz@ysfri.ac.cn.
  • Liu W; College of Chemistry and ChemicalEngineering, Ocean University of China, Qingdao 266100, People's Republic of China.
  • Lu X; School of Chemistry and The Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia.
  • Justin Gooding J; School of Chemistry and The Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia.
  • Li Q; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, People's Republic of China.
  • Qu K; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, People's Republic of China.
Anal Biochem ; 466: 16-8, 2014 Dec 01.
Article en En | MEDLINE | ID: mdl-25168192
Loop-mediated isothermal amplification (LAMP) yields a large amount of DNA, as well as magnesium pyrophosphate precipitate, causing a decrease in ionic strength that can be measured with a conductivity meter. There is a clear relationship between the conductivity of the LAMP mixture solution and the duration of biochemical reaction. Moreover, there is also a clear relationship between the change in conductivity and the amount of initial template DNA over the range of 0.08 to 3.2 ng. These results demonstrate the feasibility not only for detecting the LAMP product qualitatively but also for real-time monitoring the biochemical reaction progression quantitatively using conductivity measurements.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Técnicas de Amplificación de Ácido Nucleico / Electroquímica Idioma: En Revista: Anal Biochem Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Técnicas de Amplificación de Ácido Nucleico / Electroquímica Idioma: En Revista: Anal Biochem Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos